Patelunas D M, Agins A P, Zipkin R E, Smith J B
Temple University, Philadelphia, PA 19140.
Agents Actions. 1989 Jun;27(3-4):469-72. doi: 10.1007/BF01972855.
5(S),12(R)-dihydroxy-6-cis-8,10 trans-14-cis-eicosatetraenoic acid (LTB4) is a potent inflammatory mediator generated by human cells. A receptor assay using membranes from cultured HL-60 cells has been developed to quantitate LTB4 with a range of sensitivity from 10 pg to 5 ng. The initial metabolite of LTB4, 20-OH LTB4, has a cross reactivity of 28% while other lipoxygenase products do not significantly compete. This assay has been used to study ionomyocin-induced formation of LTB4 by human neutrophils. The use of membranes from HL-60 cells for the measurement of LTB4 provides a sensitive and highly selective alternative to radioimmunoassay for the determination of the levels of this important eicosanoid in biological fluids and should be useful in the development of antagonists of the LTB4 receptor.
5(S),12(R)-二羟基-6-顺式-8,10-反式-14-顺式二十碳四烯酸（LTB4）是人体细胞产生的一种强效炎症介质。已开发出一种使用培养的HL-60细胞膜进行的受体测定法，用于定量LTB4，灵敏度范围为10皮克至5纳克。LTB4的初始代谢产物20-OH LTB4的交叉反应率为28%，而其他脂氧合酶产物则无明显竞争。该测定法已用于研究离子霉素诱导人中性粒细胞形成LTB4的过程。使用HL-60细胞膜测量LTB4为生物流体中这种重要类花生酸水平的测定提供了一种比放射免疫测定更灵敏、更具选择性的方法，并且在LTB4受体拮抗剂的开发中应该会很有用。
