Ford-Hutchinson A W, Bray M A, Doig M V, Shipley M E, Smith M J
Nature. 1980 Jul 17;286(5770):264-5. doi: 10.1038/286264a0.
Arachidonic acid is metabolised either by cyclooxygenases to produce prostaglandins and thromboxanes or by lipoxygenases to produce mono-, di- and trihydroxyeicosatetraenoic acids (HETEs). Polymorphonuclear leukocytes (PMNs) release HETEs, including mono- and dihydroxy fatty acids, when exposed to stimuli such as the calcium ionophore A23187 (refs 1, 2). The mono-HETEs are assumed to be of particular importance with respect to effects on leukocyte function because they have been shown to possess both chemotactic and chemokinetic activities towards PMNs and eosinophils. However, we have now shown that the chemokinetic and aggregating activities released from rat and human PMNs exposed to ionophore A23187 (ref. 5) are not due to the release of mono-HETEs but to that of 5, 12-di-HETE (leukotriene B). This compound is active over the concentration range 10 pg ml-1 to 5 ng ml-1.
花生四烯酸可通过环氧化酶代谢生成前列腺素和血栓素，也可通过脂氧合酶代谢生成单羟基、二羟基和三羟基二十碳四烯酸（HETEs）。多形核白细胞（PMNs）在暴露于钙离子载体A23187等刺激物时会释放HETEs，包括单羟基和二羟基脂肪酸（参考文献1、2）。单羟基HETEs被认为对白细胞功能的影响尤为重要，因为它们已被证明对PMNs和嗜酸性粒细胞具有趋化和化学促动活性。然而，我们现在已经表明，暴露于离子载体A23187的大鼠和人PMNs释放的化学促动和聚集活性（参考文献5）并非由于单羟基HETEs的释放，而是由于5,12-二羟基HETE（白三烯B）的释放。该化合物在10 pg/ml至5 ng/ml的浓度范围内具有活性。
