González-López Santiago, Torres-Rodríguez Carolina, Bolaños-Carmona Victoria, Sanchez-Sanchez Purificación, Rodríguez-Navarro Alejandro, Álvarez-Lloret Pedro, Domingo Garcia María
Department of Pathology and Dental Therapeutics, Faculty of Dentistry, University of Granada, Campus de Cartuja, 18071, Granada, Spain.
Department of Oral Health, Faculty of Dentistry, National University of Colombia, Bogotá, Colombia.
Odontology. 2016 Jan;104(1):44-52. doi: 10.1007/s10266-014-0189-7. Epub 2014 Dec 21.
A combination of atomic absorption spectroscopy (AAS), Fourier transform infrared spectroscopy (FTIR), scanning electronic microscopy (SEM), and gas adsorption techniques was used to characterize the effect of 30 % hydrogen peroxide (HP) on enamel surface. To perform the analyses of AAS, 1 ml of 30 % HP was added to 30 mg of a bovine enamel powder sample (150-200 µm fractions) for times of 5, 20, 60, 90, and 120 min; then 5 ml of the solution was withdrawn after each time period to measure [Ca(2+)] ions. The remaining powder was recovered and analyzed by FTIR. For SEM and gas adsorption tests, 4 × 4 mm(2) enamel sectioned samples were polished and 30 % HP was applied on the surface for the same time periods. AAS data show that 30 % HP treatment mobilized calcium from the enamel at all times studied. FTIR spectra showed that the total amount of phosphate and carbonate mineral contents such as amide I decreased significantly. SEM revealed that randomly distributed areas throughout the smooth enamel surface treatment became rougher and more irregular. These alterations indicate that surface damage increases with increasing durations of HP treatment. Gas adsorption analysis proved that bleached enamel is a typically non-porous material with a small specific surface area which decreases slightly with the 30 % HP treatment. In sum, 30 % HP induced a significant alteration of the organic and mineral part of the enamel, leading to the release of calcium and a rougher, more irregular enamel surface on randomly distributed areas.
采用原子吸收光谱法（AAS）、傅里叶变换红外光谱法（FTIR）、扫描电子显微镜法（SEM）和气体吸附技术相结合的方法，来表征30%过氧化氢（HP）对牙釉质表面的影响。为进行AAS分析，将1 ml 30%的HP添加到30 mg牛牙釉质粉末样品（150 - 200 µm粒度）中，处理时间分别为5、20、60、90和120分钟；然后在每个时间段后取出5 ml溶液，测量[Ca(2+)]离子。回收剩余粉末并用FTIR进行分析。对于SEM和气体吸附测试，将4×4 mm(2)的牙釉质切片样品进行抛光，并在表面施加30%的HP，处理时间相同。AAS数据表明，在所有研究的时间点，30%的HP处理均使牙釉质中的钙流失。FTIR光谱显示，磷酸盐和碳酸盐矿物成分（如酰胺I）的总量显著减少。SEM显示，整个光滑牙釉质表面处理后的随机分布区域变得更粗糙、更不规则。这些变化表明，随着HP处理时间的增加，表面损伤加剧。气体吸附分析证明，漂白后的牙釉质是一种典型的无孔材料，比表面积小，经30%的HP处理后略有减小。总之，30%的HP导致牙釉质的有机和无机部分发生显著变化，导致钙释放，以及随机分布区域的牙釉质表面更粗糙、更不规则。
