Mirzaei Hamidreza, Djavid Gholamreza Esmaeeli, Hadizadeh Mahnaz, Jahanshiri-Moghadam Maryam, Hajian Parastoo
Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Medical Laser Research Center, Iranian Center for Medical Lasers (ICML), Academic Center for Education, Culture & Research (ACECR), Tehran, Iran.
J Photochem Photobiol B. 2015 Jan;142:86-91. doi: 10.1016/j.jphotobiol.2014.11.007. Epub 2014 Dec 9.
Photodynamic therapy (PDT) is a relatively novel modality for the treatment of cancer and some non-malignant lesions. PDT uses a photosensitive drug and light to destroy malignant cells. The aim of this study was to determine in vitro efficacy of Radachlorin-based PDT (Radachlorin-PDT) on human hepatocellular carcinoma (HCC).
The study used human liver cancer cells (HepG2) and normal liver cells (HFLF-PI4) to evaluate cell viability using the standard 2-(4, 5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The mechanism of cell death following Radachlorin-PDT was determined by DNA agarose gel electrophoresis and flow cytometry.
Radachlorin without light irradiation had no toxic effect on HepG2 and HFLF-PI4 cells. Cell survival of HepG2 and HFLF-PI4 cells were decreased following PDT in a concentration-dependent manner. However, HepG2 cells were much more sensitive to Radachlorin-PDT than HFLF-PI4 cells. Radachlorin LD50 on HepG2 cells was 30μg/ml and 20μg/ml, 24h after exposure to doses of 5J/cm(2) and 15, or 25J/cm(2), respectively. Optimal Radachlorin and light dose to kill HepG2 cells with minimal effects on normal HFLF-PI4 cells were 100μg/ml and 15J/cm(2), respectively. Our results also showed that apoptosis is induced in HepG2 cells following Radachlorin-PDT.
Our in vitro data suggest that the use of PDT with Radachlorin can be effective in the treatment of HCC.
光动力疗法（PDT）是一种相对新颖的癌症及某些非恶性病变的治疗方式。PDT利用一种光敏药物和光来破坏恶性细胞。本研究的目的是确定基于二氢卟吩e6的光动力疗法（二氢卟吩e6 - PDT）对人肝细胞癌（HCC）的体外疗效。
本研究使用人肝癌细胞（HepG2）和正常肝细胞（HFLF - PI4），采用标准的2 -（4,5 - 二甲基 - 2 - 噻唑基）- 3,5 - 二苯基 - 2H - 四氮唑溴盐（MTT）法评估细胞活力。通过DNA琼脂糖凝胶电泳和流式细胞术确定二氢卟吩e6 - PDT后细胞死亡的机制。
无光照射时，二氢卟吩e6对HepG2和HFLF - PI4细胞无毒性作用。PDT后，HepG2和HFLF - PI4细胞的存活率呈浓度依赖性降低。然而，HepG2细胞对二氢卟吩e6 - PDT比HFLF - PI4细胞敏感得多。在分别暴露于5J/cm²和15J/cm²或25J/cm²剂量24小时后，二氢卟吩e6对HepG2细胞的半数致死剂量分别为30μg/ml和20μg/ml。以对正常HFLF - PI4细胞影响最小的剂量杀死HepG2细胞的最佳二氢卟吩e6和光剂量分别为100μg/ml和15J/cm²。我们的结果还表明，二氢卟吩e6 - PDT后HepG2细胞发生凋亡。
我们的体外数据表明，使用二氢卟吩e6进行光动力疗法可有效治疗肝癌。
