Meng Hao-Tian, Zhang Li-Ping, Wu Hua, Yang Chun-Hua, Chen Jian-Gang, Wang Yan, Yan Jiang-Wei, Wang Hong-Dan, Zhang Yu-Dang, Liu Wen-Juan, Zhu Bo-Feng
Research Center of Stomatology, Stomatological Hospital, Xi'an Jiaotong University, Xi'an, PR China; Xi'an Jiaotong University Health Science Center, Xi'an, PR China.
Biochemistry and Molecular Biology Department, College of Medicine, Xinjiang Medical University, Urumqi, PR China.
Gene. 2015 Feb 25;557(2):222-8. doi: 10.1016/j.gene.2014.12.037. Epub 2014 Dec 18.
In the present study, we investigated the genetic polymorphisms of 20 novel STR loci and one previously studied locus in the Xibe ethnic group from China, as well as its genetic relationships with neighboring populations. Totally 226 unrelated healthy Xibe individuals were involved in the study. At least 5 alleles were observed for each locus, with the minimum and maximum allelic frequencies of 0.0022 and 0.5221, respectively. We obtained the lowest and highest observed heterozygosity and expected heterozygosity at locus D1S1627 and D19S433, respectively. The values of combined power of discrimination and probability of exclusion of all the 21 STR loci were 0.99999999999999999997310 and 0.999998650, respectively. Analyses of interpopulation differentiation, principal component analysis, genetic distance and phylogenetic tree revealed the relationships between Xibe group and its neighboring groups, showing that the studied Xibe group had a close genetic relationship with the Mongolian group. The present results indicated that these 21 STR loci had high genetic polymorphisms in the studied Xibe group, and were capable for the paternity testing and individual identification in forensic application.
在本研究中，我们调查了中国锡伯族中20个新的STR基因座和1个先前已研究的基因座的遗传多态性，以及其与相邻群体的遗传关系。共有226名无亲缘关系的健康锡伯族个体参与了本研究。每个基因座至少观察到5个等位基因，最小和最大等位基因频率分别为0.0022和0.5221。我们分别在基因座D1S1627和D19S433处获得了最低和最高的观察杂合度与期望杂合度。所有21个STR基因座的联合鉴别力和排除概率值分别为0.99999999999999999997310和0.999998650。群体间分化分析、主成分分析、遗传距离和系统发育树揭示了锡伯族群体与其相邻群体之间的关系，表明所研究的锡伯族群体与蒙古族群体有密切的遗传关系。目前的结果表明，这21个STR基因座在所研究的锡伯族群体中具有高度的遗传多态性，并且能够用于法医应用中的亲子鉴定和个体识别。
