Scientific Research and Experiment Center, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710004, People's Republic of China.
Mol Biol Rep. 2012 Dec;39(12):10265-71. doi: 10.1007/s11033-012-1903-6. Epub 2012 Oct 13.
The aim of this study was to investigate allelic frequency distribution and forensic genetic parameters of autosomal short tandem repeats (STR) loci of the population samples from 107 Tujia individuals from Chinese Hubei Province. Twenty-one autosomal STR genetic markers (D9S1122, D6S474, D6S1017, D5S2500, D4S2408, D3S4529, D2S441, D2S1776, D22S1045, D20S482, D1S1677, D1S1627, D1GATA113, D19S433, D18S853, D17S1301, D11S4463, D12ATA63, D10S1248, D10S1435 and D14S1434) were simultaneously amplified in a new multiplex polymerase chain reaction system. 155 alleles for all the STR loci from the Tujia population were observed and the corresponding allelic frequencies ranged from 0.005 to 0.589. Expected heterozygosity, polymorphic information content, power of discrimination and power of exclusion of the 21 STR loci in the Tujia population were from 0.579 to 0.824, from 0.525 to 0.802, from 0.773 to 0.945 and from 0.257 to 0.641, respectively. Our results indicate that the autosomal STRs multiplex system provides highly informative STR data and could be useful in forensic individual identification and parentage testing in this region.
本研究旨在调查来自中国湖北省 107 名土家族人群样本的常染色体短串联重复序列（STR）基因座的等位基因频率分布和法医遗传学参数。该研究使用 21 个常染色体 STR 遗传标记（D9S1122、D6S474、D6S1017、D5S2500、D4S2408、D3S4529、D2S441、D2S1776、D22S1045、D20S482、D1S1677、D1S1627、D1GATA113、D19S433、D18S853、D17S1301、D11S4463、D12ATA63、D10S1248、D10S1435 和 D14S1434）在一个新的多重聚合酶链反应系统中同时扩增。从土家族人群中观察到所有 STR 基因座的 155 个等位基因，相应的等位基因频率范围从 0.005 到 0.589。21 个 STR 基因座在土家族人群中的预期杂合度、多态信息含量、鉴别力和排除力分别为 0.579-0.824、0.525-0.802、0.773-0.945 和 0.257-0.641。我们的研究结果表明，常染色体 STR 多重系统提供了高度信息性的 STR 数据，可用于该地区的法医个体识别和亲子关系鉴定。
