Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, 710004, P. R. China.
Clinical Reaserch Center of Shaanxi Province for Dental and Maxillofacial diseases, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, 710004, P. R. China.
Sci Rep. 2017 Mar 22;7:44407. doi: 10.1038/srep44407.
We analyzed the genetic polymorphisms of 54 mitochondrial DNA (mtDNA) variants in Chinese Xibe ethnic minority group. A total of 137 unrelated healthy volunteers from Chinese Xibe group were the objects of our study. Among the selected loci, there were 51 variable positions including transitions and transversions, and single nucleotide transitions were common (83.93%) versus transversions. These variations defined 64 different mtDNA haplotypes exclusive of (CA) and 9 bp deletion variation. The haplotype diversity and discrimination power in Xibe population were 0.9800 ± 0.004 and 0.9699, respectively. Besides, we compared Xibe group with 18 other populations and reconstructed a phylogenetic tree using Neighbor-Joining method. The result revealed that Xibe group was a close to Xinjiang Han and Yanbian Korean groups. Our data also indicated that Xibe group has a close relationship with Daur and Ewenki groups, which is reflected by the history that Xibe was influenced by Daur and Ewenki groups during the development of these groups. In conclusion, the variants we studied are polymorphic and could be used as informative genetic markers for forensic and population genetic application.
我们分析了中国锡伯族群体中 54 个线粒体 DNA（mtDNA）变体的遗传多态性。我们的研究对象是来自中国锡伯族的 137 名无关健康志愿者。在所选择的基因座中，有 51 个变异位置，包括转换和颠换，单核苷酸转换比颠换更为常见（83.93%）。这些变异定义了 64 种不同的 mtDNA 单倍型，不包括（CA）和 9bp 缺失变异。锡伯族群体的单倍型多样性和辨别力分别为 0.9800±0.004 和 0.9699。此外，我们将锡伯族群体与其他 18 个群体进行了比较，并使用邻接法构建了系统发生树。结果表明，锡伯族群体与新疆汉族和延边朝鲜族群体关系密切。我们的数据还表明，锡伯族群体与达斡尔族和鄂温克族群体关系密切，这反映了在这些群体的发展过程中，锡伯族受到了达斡尔族和鄂温克族的影响。总之，我们研究的变体是多态的，可以作为法医学和群体遗传学应用的信息遗传标记。
