Johnson A J, Shukle R H, Chen M-S, Srivastava S, Subramanyam S, Schemerhorn B J, Weintraub P G, Abdel Moniem H E M, Flanders K L, Buntin G D, Williams C E
USDA-ARS, Crop Production and Pest Control Research Unit, West Lafayette, IN, USA; Department of Entomology, Purdue University, West Lafayette, IN, USA.
Insect Mol Biol. 2015 Apr;24(2):191-202. doi: 10.1111/imb.12148. Epub 2014 Dec 22.
Evidence is emerging that some proteins secreted by gall-forming parasites of plants act as effectors responsible for systemic changes in the host plant, such as galling and nutrient tissue formation. A large number of secreted salivary gland proteins (SSGPs) that are the putative effectors responsible for the physiological changes elicited in susceptible seedling wheat by Hessian fly, Mayetiola destructor (Say), larvae have been documented. However, how the genes encoding these candidate effectors might respond under field conditions is unknown. The goal of this study was to use microarray analysis to investigate variation in SSGP transcript abundance amongst field collections from different geographical regions (southeastern USA, central USA, and the Middle East). Results revealed significant variation in SSGP transcript abundance amongst the field collections studied. The field collections separated into three distinct groups that corresponded to the wheat classes grown in the different geographical regions as well as to recently described Hessian fly populations. These data support previous reports correlating Hessian fly population structure with micropopulation differences owing to agro-ecosystem parameters such as cultivation of regionally adapted wheat varieties, deployment of resistance genes and variation in climatic conditions.
越来越多的证据表明，植物瘿瘤形成寄生虫分泌的一些蛋白质作为效应子，负责宿主植物的系统性变化，如瘿瘤形成和营养组织形成。大量分泌唾液腺蛋白（SSGPs）被记录为可能的效应子，它们负责由黑森瘿蚊（Mayetiola destructor (Say)）幼虫在易感小麦幼苗中引发的生理变化。然而，编码这些候选效应子的基因在田间条件下如何反应尚不清楚。本研究的目的是使用微阵列分析来研究来自不同地理区域（美国东南部、美国中部和中东）的田间采集样本中SSGP转录本丰度的变化。结果显示，在所研究的田间采集样本中，SSGP转录本丰度存在显著差异。田间采集样本分为三个不同的组，这与在不同地理区域种植的小麦品种以及最近描述的黑森瘿蚊种群相对应。这些数据支持了先前的报告，即由于农业生态系统参数，如区域适应性小麦品种的种植、抗性基因的部署和气候条件的变化，黑森瘿蚊种群结构与微种群差异相关。
