Demiaszkiewicz Aleksander W, Polańczyk Grażyna, Osińska Barbara, Pyziel Anna M, Kuligowska Izabela, Lachowicz Jacek, Sikorski Adam
W. Stefański Institute of Parasitology, Polish Academy of Sciences, Warsaw, Poland.
'Megawet', Veterinary Clinic, Milanówek, Poland.
Ann Agric Environ Med. 2014;21(4):701-4. doi: 10.5604/12321966.1129918.
The aim of this study was to determine the prevalence and distribution of canine dirofilariosis from different districts of the Mazovian Province in central-eastern Poland. 462 dogs aged from 1.5-14 years were examined for dirofilariosis. Blood samples were examined for the presence of microfilariae using the Knott method, as well as the method of Kingston and Morton, after centrifugation in haematocrit microtubes in order to determine the intensity of infection as the number of microfilariae in 60 microliters of blood. The species of microfilariae found were determined after staining on the basis of the morphological characters. Samples were also examined using Canine Heartworm Antigen Test Kit SNAP HTWM (IDEXX, USA) that allows the detection of circulating antigens of females of Dirofilaria immitis. The positive samples were examined using multiplex PCR assay for species confirmation. Microfilariae belonging to the species D. repens were found in the blood samples of dogs originating from the city of Warsaw and from 18 districts of Mazovian Province. The mean prevalence of that species observed in the province was 25.8%. The range of intensity counted with the number of microfilariae found in 60 microliters of blood amounted to between 1-150; median intensity 9 microfilariae. The highest prevalence, reaching 52.9% and the highest median intensity of infection reaching 28 microfilariae, was found in Radom district. The lowest prevalence, amounting to 4.2%, was registered in Grójec district; however, the lowest median intensity reaching 6 microfilariae was noted in Żyrardów district. The infected dogs had not been outside of Poland, which means that this is an autochthonous infection. Microfilariae and antigens of D. immitis were not found in any examined blood samples. Results of the presented study show that autochthonous dirofilariosis caused by nematodes D. repens commonly occurs in the area of the Mazovian Province.
本研究的目的是确定波兰中东部马佐夫舍省不同地区犬恶丝虫病的流行情况和分布。对462只年龄在1.5至14岁之间的犬进行了恶丝虫病检查。血液样本采用诺特氏法以及金斯顿和莫顿氏法进行微丝蚴检测，即在血细胞比容微量管中离心后进行检测，以确定感染强度，即每60微升血液中的微丝蚴数量。根据形态特征对染色后发现的微丝蚴种类进行鉴定。样本还使用犬心丝虫抗原检测试剂盒SNAP HTWM（美国IDEXX公司）进行检测，该试剂盒可检测犬恶丝虫雌虫的循环抗原。对阳性样本进行多重PCR检测以确认种类。在来自华沙市和马佐夫舍省18个地区的犬的血液样本中发现了属于匐行恶丝虫的微丝蚴。该省观察到的该物种平均流行率为25.8%。以每60微升血液中发现的微丝蚴数量计算的感染强度范围为1至150；中位强度为9条微丝蚴。在拉多姆区发现了最高流行率，达到52.9%，最高中位感染强度达到28条微丝蚴。在格罗耶茨区记录到最低流行率，为4.2%；然而，在热拉尔多夫区观察到最低中位强度，为6条微丝蚴。受感染的犬未曾离开过波兰，这意味着这是一种本土感染。在任何检测的血液样本中均未发现犬恶丝虫的微丝蚴和抗原。本研究结果表明，由匐行恶丝虫线虫引起的本土恶丝虫病在马佐夫舍省地区普遍存在。
