Huang Yihua, Jiang Hongwei, Gong Qimei, Li Xuyan, Ling Junqi
Guangdong Provincial Key Laboratory of Stomatology, Department of Operative Dentistry and Endodontics, Guanghua School and Hospital of Stomatology, Sun Yat‑sen University, Guangzhou, Guangdong 510055, P.R. China.
Mol Med Rep. 2015 May;11(5):3547-52. doi: 10.3892/mmr.2014.3120. Epub 2014 Dec 19.
Lipopolysaccharide (LPS) is one of the causative agents of pulpitis and previous studies have demonstrated that the LPS stimulation of human aortic valve interstitial cells induces inflammatory mediators and the gene expression of osteogenic factors. Therefore, in the present study, it was hypothesized that LPS affects the odontoblastic differentiation of human dental pulp cells (hDPCs). In order to investigate this, an in vitro study using hDPCs was performed. Increased alkaline phosphatase (ALP) activity was observed in the hDPCs treated with LPS, which was more marked when the cells were costimulated with odontogenic induction medium (OM). LPS also appeared to increase the gene expression levels of dentin sialophosphoprotein and dentin matrix protein‑1 and the protein expression level of dental sialoprotein in the hDPCs, particularly in combination with OM. In addition, the size and the number of nodules formed in the hDPCs exposed to OM and LPS were increased compared to those stimulated by OM alone. To determine the role of nuclear factor κB (NF‑κB) during the LPS‑induced odontoblastic differentiation of hDPCs, immunofluorescence was performed. The nuclear translocation of NF‑κB, induced by LPS was confirmed, suggesting its involvement in the LPS‑induced increase in odontoblastic differentiation of hDPCs. In conclusion, there may be an association between LPS stimulation, with or without OM, and odontoblastic differentiation.
脂多糖（LPS）是牙髓炎的致病因素之一，先前的研究表明，LPS刺激人主动脉瓣间质细胞可诱导炎症介质和成骨因子的基因表达。因此，在本研究中，我们假设LPS会影响人牙髓细胞（hDPCs）的成牙本质细胞分化。为了对此进行研究，我们使用hDPCs进行了一项体外研究。在用LPS处理的hDPCs中观察到碱性磷酸酶（ALP）活性增加，当细胞与成牙诱导培养基（OM）共同刺激时，这种增加更为明显。LPS似乎还增加了hDPCs中牙本质涎磷蛋白和牙本质基质蛋白-1的基因表达水平以及牙涎蛋白的蛋白表达水平，特别是在与OM联合使用时。此外，与单独用OM刺激的hDPCs相比，暴露于OM和LPS的hDPCs中形成的结节大小和数量增加。为了确定核因子κB（NF-κB）在LPS诱导的hDPCs成牙本质细胞分化过程中的作用，我们进行了免疫荧光实验。证实了LPS诱导的NF-κB核转位，表明其参与了LPS诱导的hDPCs成牙本质细胞分化增加。总之，LPS刺激（有无OM）与成牙本质细胞分化之间可能存在关联。
