Kim Jae-Hwan, Woo Su-Mi, Choi Nam-Ki, Kim Won-Jae, Kim Seon-Mi, Jung Ji-Yeon
Department of Pediatric Dentistry, Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, South Korea.
Department of Oral Physiology, Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, South Korea; Research Center for Biomineralization Disorder, Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, South Korea.
J Endod. 2017 Mar;43(3):433-438. doi: 10.1016/j.joen.2016.11.002.
Platelet-rich fibrin (PRF), as an autologous fibrin matrix, is known to contain platelets, leukocytes, and growth factors to control inflammation and to facilitate the healing process. The purpose of this study was to investigate the effects of PRF on odontoblastic differentiation in human dental pulp cells (HDPCs) treated with lipopolysaccharide (LPS).
Gene expression of inflammatory cytokines and adhesion molecules on the HDPCs cultured with or without LPS and PRF extract (PRFe) were evaluated by reverse-transcription polymerase chain reaction and Western blot analysis. In addition, odontoblastic differentiation was determined by measuring alkaline phosphatase (ALP) activity using ALP staining, the expression of odontogenesis-related genes, and the extent of mineralization using alizarin red S staining.
Treatment with PRFe significantly attenuated the LPS-stimulated expression of interleukin (IL)-1β, IL-6, and IL-8 in HDPCs. In addition, PRFe inhibited the up-regulation of vascular cell adhesion molecule 1 and the production of intracellular adhesion molecule 1 in HDPCs exposed to LPS. Expression of dentin sialophosphoprotein and dentin matrix acidic phosphoprotein 1, ALP activity, and mineralization were enhanced by PRFe in LPS-treated HDPCs.
These results suggest that PRF has effects associated not only with inhibition of inflammation in HDPCs exposed to LPS but also stimulation of odontoblastic differentiation.
富含血小板纤维蛋白（PRF）作为一种自体纤维蛋白基质，已知含有血小板、白细胞和生长因子，可控制炎症并促进愈合过程。本研究的目的是探讨PRF对用脂多糖（LPS）处理的人牙髓细胞（HDPCs）成牙本质细胞分化的影响。
通过逆转录聚合酶链反应和蛋白质免疫印迹分析评估在有或无LPS和PRF提取物（PRFe）的情况下培养的HDPCs上炎症细胞因子和黏附分子的基因表达。此外，通过使用碱性磷酸酶（ALP）染色测量ALP活性、牙胚发生相关基因的表达以及使用茜素红S染色测量矿化程度来确定成牙本质细胞分化。
用PRFe处理可显著减弱LPS刺激的HDPCs中白细胞介素（IL）-1β、IL-6和IL-8的表达。此外，PRFe抑制暴露于LPS的HDPCs中血管细胞黏附分子1的上调和细胞间黏附分子1的产生。PRFe增强了LPS处理的HDPCs中牙本质涎磷蛋白和牙本质基质酸性磷酸蛋白1的表达、ALP活性以及矿化。
这些结果表明，PRF不仅具有抑制暴露于LPS的HDPCs中炎症的作用，还具有刺激成牙本质细胞分化的作用。
