Schneider T, Siegemund T, Siegemund R, Petros S
Dr. Sirak Petros, University Hospital Leipzig, Medical ICU, Liebigstr. 20, 04103 Leipzig, Germany, Tel. +49/(0)341/97 12 70-0, Fax -9, E-mail:
Hamostaseologie. 2015;35(2):181-6. doi: 10.5482/HAMO-14-08-0033. Epub 2014 Dec 22.
Published data on thrombin generation variables and their correlation with thromboelastometry in the healthy population are scarce. This study aimed at assessing thrombin generation in adults and its correlation to classical rotational thromboelastometry (ROTEM).
Thrombin generation was measured in platelet-poor plasma from healthy volunteers using the calibrated automated thrombogram (CAT) with 1 and 5 pmol/l tissue factor final concentration. Lag time, thrombin peak, time to thrombin peak and endogenous thrombin potential (ETP) were analyzed. ROTEM was performed without activator (NATEM) and data for clotting time, alpha angle, clot formation time and maximum clot firmness were correlated with those of thrombin generation.
Altogether 132 persons (72 men, 60 women; median age: 48.0 years) were included. There was a positive non-linear correlation for age versus lag time (p < 0.001) and time to peak (p = 0.001), and almost linear correlation for age versus thrombin peak (p = 0.024) and ETP (p = 0.001), although with a moderate regression slope. Regarding ROTEM, there was a positive correlation between age and maximum clot firmness and alpha angle (p = 0.001), but a negative correlation between age and clotting time (p = 0.039). Comparing both assays, thrombin peak and ETP measured with a final tissue factor concentration of 5 pmol/l correlated significantly with alpha angle and maximum clot firmness.
The age-related changes in CAT and ROTEM variables among adults are not linear. There is a significant correlation, although with a moderate slope, between data from CAT measured with 5 pmol/l tissue factor and ROTEM.
关于健康人群凝血酶生成变量及其与血栓弹力图相关性的已发表数据稀缺。本研究旨在评估成年人的凝血酶生成情况及其与经典旋转血栓弹力图（ROTEM）的相关性。
使用校准自动凝血图（CAT），在最终组织因子浓度为1和5 pmol/l的情况下，测量健康志愿者乏血小板血浆中的凝血酶生成。分析滞后时间、凝血酶峰值、达到凝血酶峰值的时间和内源性凝血酶潜力（ETP）。在不使用激活剂的情况下进行ROTEM（NATEM），并将凝血时间、α角、凝血形成时间和最大凝块硬度的数据与凝血酶生成数据进行相关性分析。
共纳入132人（72名男性，60名女性；中位年龄：48.0岁）。年龄与滞后时间（p < 0.001）和达到峰值的时间（p = 0.001）呈正非线性相关，年龄与凝血酶峰值（p = 0.024）和ETP（p = 0.001）呈几乎线性相关，尽管回归斜率适中。关于ROTEM，年龄与最大凝块硬度和α角呈正相关（p = 0.001），但年龄与凝血时间呈负相关（p = 0.039）。比较两种检测方法，最终组织因子浓度为5 pmol/l时测得的凝血酶峰值和ETP与α角和最大凝块硬度显著相关。
成年人中CAT和ROTEM变量随年龄的变化并非线性。使用5 pmol/l组织因子测量的CAT数据与ROTEM数据之间存在显著相关性，尽管斜率适中。
