Bräunlich H, Pfeifer R, Grau P, Reznik L
Institute of Pharmacology and Toxicology, Friedrich Schiller University Jena, DDR.
Biomed Biochim Acta. 1989;48(7):457-64.
In conscious rats, administration of vanadate is followed by a distinct and statistically significant diuretic effect. The increase in renal excretion of sodium dominates in comparison with other electrolytes. After i.p. administration vanadate is effective for 30 to 60 min. In anesthetized rats an excessive saline load and i.v. administration of vanadate do not prolong the renal effectiveness of vanadate. A long lasting diuretic effect is measurable during a constant infusion of vanadate. p-Aminohippurate transport in renal cortical slices can be inhibited by adding vanadate to the incubation medium. Vanadate administered in vivo in diuretically effective doses influences the p-aminohippurate transport in consectively prepared renal cortical slices. A 50% inhibition of Na+/K+-ATPase can be provoked by i.p. administration of 0.6 mg vanadate/100 g b.m. whereas the maximal increase in renal excretion of sodium is measurable following 0.5 mg vanadate/100 g b.m.
在清醒大鼠中，给予钒酸盐后会出现明显且具有统计学意义的利尿作用。与其他电解质相比，钠的肾排泄增加占主导。腹腔注射钒酸盐后，其作用可持续30至60分钟。在麻醉大鼠中，过量的盐水负荷和静脉注射钒酸盐并不会延长钒酸盐的肾脏作用效果。在持续输注钒酸盐期间可检测到持久的利尿作用。向孵育介质中添加钒酸盐可抑制肾皮质切片中的对氨基马尿酸转运。以利尿有效剂量在体内给予钒酸盐会影响连续制备的肾皮质切片中的对氨基马尿酸转运。腹腔注射0.6毫克钒酸盐/100克体重可引起Na+/K+-ATP酶50%的抑制，而在注射0.5毫克钒酸盐/100克体重后可检测到钠的肾排泄量最大增加。
