Goudarzi Saeid, Ahmadi Anita, Farhadi Mohammad, Kamrava Seyed Kamran, Saghafi Shiva, Omidfar Kobra
Clinical Nanomedicine Laboratory, ENT- Head & Neck Research Center, Hazrate Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.
Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, and Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Allergy Asthma Immunol. 2015 Feb;14(1):105-12.
A new competitive immunochromatography (ICG) strip test based on polyclonal antibody (pAb) conjugated with gold nanoparticles (NPs) was developed and its applications for primary screening of immunoglobulin (Ig) A in serum were evaluated. Nanocolloidal gold as the detection reagent, with an average particle diameter of 20 nm, was synthesized and labelled pAb. The antibody-nanocolloidal gold probe was applied on the conjugate pad, and human IgA was immobilized on a nitrocellulose membrane as the capture reagent to prepare the ICG strip test. It took only 10 minutes to accomplish a semi-quantitative detection of serum IgA in this assay. In the optimized investigational conditions, the ICG strip test could distinguish human serum IgA in the range from 1 to 270 ng/mL with a detection limit of 5 ng/mL. The reliability of testing procedures was examined by performing the ICG strip test with 11 serum samples and comparing the results with those obtained via enzyme-linked immunosorbent assay (ELISA). The ICG strip was sufficiently sensitive and accurate for a rapid screening of IgA in human serum.
开发了一种基于与金纳米颗粒（NPs）偶联的多克隆抗体（pAb）的新型竞争性免疫层析（ICG）试纸条检测方法，并评估了其在血清免疫球蛋白（Ig）A初筛中的应用。合成了平均粒径为20nm的纳米胶体金作为检测试剂，并标记pAb。将抗体-纳米胶体金探针应用于结合垫上，将人IgA固定在硝酸纤维素膜上作为捕获试剂，制备ICG试纸条检测方法。该检测方法仅需10分钟即可完成血清IgA的半定量检测。在优化的研究条件下，ICG试纸条检测方法能够区分浓度范围为1至270ng/mL的人血清IgA，检测限为5ng/mL。通过对11份血清样本进行ICG试纸条检测，并将结果与酶联免疫吸附测定（ELISA）获得的结果进行比较，检验了检测程序的可靠性。ICG试纸条对于快速筛查人血清中的IgA具有足够的敏感性和准确性。
