Varghese George M, Janardhanan Jeshina, Mahajan Sanjay K, Tariang David, Trowbridge Paul, Prakash John A J, David Thambu, Sathendra Sowmya, Abraham O C
Emerg Infect Dis. 2015 Jan;21(1):64-9. doi: 10.3201/eid2101.140580.
Scrub typhus, an acute febrile illness that is widespread in the Asia-Pacific region, is caused by the bacterium Orientia tsutsugamushi, which displays high levels of antigenic variation. We conducted an investigation to identify the circulating genotypes of O. tsutsugamushi in 3 scrub typhus-endemic geographic regions of India: South India, Northern India, and Northeast India. Eschar samples collected during September 2010-August 2012 from patients with scrub typhus were subjected to 56-kDa type-specific PCR and sequencing to identify their genotypes. Kato-like strains predominated (61.5%), especially in the South and Northeast, followed by Karp-like strains (27.7%) and Gilliam and Ikeda strains (2.3% each). Neimeng-65 genotype strains were also observed in the Northeast. Clarifying the genotypic diversity of O. tsutsugamushi in India enhances knowledge of the regional diversity among circulating strains and provides potential resources for future region-specific diagnostic studies and vaccine development.
恙虫病是一种在亚太地区广泛传播的急性发热性疾病，由恙虫病东方体引起，该病原体具有高度的抗原变异。我们开展了一项调查，以确定印度3个恙虫病流行地理区域（南印度、北印度和东北印度）中恙虫病东方体的流行基因型。2010年9月至2012年8月期间从恙虫病患者身上采集的焦痂样本进行了56 kDa型特异性PCR和测序，以确定其基因型。加藤样菌株占主导地位（61.5%），尤其是在南部和东北部，其次是卡尔普样菌株（27.7%）以及吉列姆和池田菌株（各占2.3%）。在东北部也观察到了内蒙-65基因型菌株。明确印度恙虫病东方体的基因型多样性，可增进对流行菌株区域多样性的了解，并为未来特定区域的诊断研究和疫苗开发提供潜在资源。
