Feeney Audrey, Johnston Christopher D, Lucid Alan, O'Mahony Jim, Coffey Aidan, Lucey Brigid, Sleator Roy D
Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork Ireland.
Gut Pathog. 2014 Dec 16;6(1):46. doi: 10.1186/s13099-014-0046-9. eCollection 2014.
We investigate the role of the C-terminal coiled coil of the secondary proline porter ProP in contributing to Cronobacter sakazakii osmotolerance.
The extended C-terminal domain of ProP1 (encoded by ESA_02131) was spliced onto the truncated C-terminal end of ProP2 (encoded by ESA_01706); creating a chimeric protein (ProPc) which exhibits increased osmotolerance relative to the wild type.
It appears that the C-terminal coiled coil domain tunes ProP at low osmolality, whereas ProP transporters lacking the coiled coil domain are more active at a higher osmolality range.
我们研究了次级脯氨酸转运蛋白ProP的C末端卷曲螺旋在阪崎肠杆菌耐渗透压方面的作用。
将ProP1（由ESA_02131编码）的延伸C末端结构域拼接至ProP2（由ESA_01706编码）的截短C末端；产生了一种嵌合蛋白（ProPc），其相对于野生型表现出更高的耐渗透压性。
似乎C末端卷曲螺旋结构域在低渗透压下调节ProP，而缺乏卷曲螺旋结构域的ProP转运蛋白在较高渗透压范围内更具活性。
