阪崎肠杆菌BAA - 894基因组的功能筛选揭示了ProP（ESA_02131）在肉碱摄取中的作用。
Functional Screening of the Cronobacter sakazakii BAA-894 Genome reveals a role for ProP (ESA_02131) in carnitine uptake.
作者信息
Feeney Audrey, Sleator Roy D
机构信息
a Department of Biological Sciences ; Cork Institute of Technology ; Bishopstown , Cork , Ireland.
出版信息
Bioengineered. 2015;6(3):161-5. doi: 10.1080/21655979.2015.1043500. Epub 2015 Apr 27.
Abstract
Cronobacter sakazakii is a neonatal pathogen responsible for up to 80% of fatalities in infected infants. Low birth weight infants and neonates infected with C. sakazakii suffer necrotizing enterocolitis, bacteraemia and meningitis. The mode of transmission most often associated with infection is powdered infant formula (PIF) which, with an aw of ∼0.2, is too low to allow most microorganisms to persist. Survival of C. sakazakii in environments subject to extreme hyperosmotic stress has previously been attributed to the uptake of compatible solutes including proline and betaine. Herein, we report the construction and screening of a C. sakazakii genome bank and the identification of ProP (ESA_02131) as a carnitine uptake system.
摘要
阪崎肠杆菌是一种新生儿病原体，在受感染婴儿中导致高达80%的死亡。低体重婴儿和感染阪崎肠杆菌的新生儿会患坏死性小肠结肠炎、菌血症和脑膜炎。最常与感染相关的传播方式是婴儿配方奶粉（PIF），其水分活度约为0.2，过低以至于大多数微生物无法存活。阪崎肠杆菌在极端高渗应激环境中的存活此前被认为归因于包括脯氨酸和甜菜碱在内的相容性溶质的摄取。在此，我们报告了阪崎肠杆菌基因组文库的构建和筛选，并鉴定出ProP（ESA_02131）为一种肉碱摄取系统。
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