Daniela Echenique, Alejandra Chiaramello, Pedro Rossomando, Claudia Mattana, Lucía Alcaráz, Carlos Tonn, Analía Laciar, Sara Satorres
Department of Biochemistry and Biological Sciences, National University of San Luis, 5700 San Luis, Argentina.
Department of Chemistry, National University of San Luis, 5700 San Luis, Argentina.
ScientificWorldJournal. 2014;2014:342143. doi: 10.1155/2014/342143. Epub 2014 Nov 3.
Nasal carriers of Staphylococcus aureus are important reservoirs with risk of developing endogenous infections or transmitting infections to susceptible individuals. Methicillin-resistant S. aureus (MRSA) are associated with higher rates of treatment failure. Some strains of S. aureus produce slime which is believed to make the microorganisms more resistant to antibiotics and host defenses. The antibacterial activity of ethyl acetate:n-hexane (EtOAc:HEX) extracts of Mulinum spinosum (5:95% EtOAc:HEX, 50:50% EtOAc:HEX, 70:30% EtOAc:HEX and mix 20:80/30:70% EtOAc:HEX, 50:50/70:30/100:0% EtOAc:HEX) were assayed against 3 slime-producing S. aureus strains and 2 MRSA strains isolated from nasal carriers. S. aureus ATCC 35556 slime-producing strain and MRSA ATCC 43300 strain were used as controls. The extracts were prepared using flash chromatography. M. spinosum 5:95% AcOEt:HEX showed antibacterial effect against all slime-producing strains (MIC: 500 µg/mL) and the highest activity against MRSA strains (MIC: 500 to 1000 µg/mL). All M. spinosum extracts assayed were active against slime-producing S. aureus and MRSA at doses between 500 and 4000 µg/mL. Both, slime-producing S. aureus and MRSA are highly contagious and hardly eradicated by antibiotic therapies. So, there is an increasing need to find new substances with the ability to inhibit these strains.
金黄色葡萄球菌的鼻腔携带者是重要的感染源，有发生内源性感染或将感染传播给易感个体的风险。耐甲氧西林金黄色葡萄球菌（MRSA）与更高的治疗失败率相关。一些金黄色葡萄球菌菌株会产生黏液，据信这种黏液会使微生物对抗生素和宿主防御更具抵抗力。对多刺木根的乙酸乙酯：正己烷（EtOAc:HEX）提取物（5:95% EtOAc:HEX、50:50% EtOAc:HEX、70:30% EtOAc:HEX以及混合的20:80/30:70% EtOAc:HEX、50:50/70:30/100:0% EtOAc:HEX）针对3株产黏液金黄色葡萄球菌菌株和2株从鼻腔携带者分离出的MRSA菌株进行了抗菌活性测定。金黄色葡萄球菌ATCC 35556产黏液菌株和MRSA ATCC 43300菌株用作对照。提取物采用快速柱色谱法制备。多刺木根5:95%乙酸乙酯：正己烷提取物对所有产黏液菌株均显示出抗菌作用（最低抑菌浓度：500μg/mL），对MRSA菌株的活性最高（最低抑菌浓度：500至1000μg/mL）。所有测定的多刺木根提取物在500至4000μg/mL的剂量下对产黏液金黄色葡萄球菌和MRSA均有活性。产黏液金黄色葡萄球菌和MRSA均具有高度传染性，且很难通过抗生素疗法根除。因此，越来越需要找到能够抑制这些菌株的新物质。
