Marino Andreana, Bellinghieri Valentina, Nostro Antonia, Miceli Natalizia, Taviano Maria Fernanda, Güvenç Ayşegül, Bisignano Giuseppe
Pharmaco-Biological Department, University of Messina, Messina, Italy.
FEMS Immunol Med Microbiol. 2010 Aug;59(3):470-6. doi: 10.1111/j.1574-695X.2010.00705.x. Epub 2010 May 28.
Methanol and aqueous branch extracts of five Juniperus species were examined for their effects on Staphylococcus aureus ATCC 6538P and S. aureus 810 biofilm. The Turkish plant material was Juniperus communis L. var. communis, J. communis L. var. saxatilis Pall., Juniperus drupacea Labill., Juniperus oxycedrus L. ssp. oxycedrus, J. oxycedrus L. ssp. macrocarpa (Sibth. & Sm.) Ball. The Juniperus extracts were subjected to preliminary phytochemical analysis by thin-layer chromatography. The antimicrobial activity was evaluated using the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The effects of the extracts on biofilm formation and preformed biofilm were quantified by both biomass OD and the CFU counting method. The phytochemical screening revealed the presence of polyphenols, coumarins, lignans, steroids, alkaloids and terpenes. For both strains, the MICs of all extracts were in the range of 4.88-78.12 microg mL(-1). On S. aureus ATCC 6538P, the effects of subinhibitory concentration (0.5 MIC) of the extracts were minimal on planktonic growth and on adhering cells, whereas they were greater on biofilm formation. Differently, on S. aureus 810, they showed only a rather low efficacy on biofilm formation. The extracts at 2 MIC demonstrated a good activity on a preformed biofilm of S. aureus ATCC 6538P.
研究了5种刺柏属植物的甲醇提取物和水提取物对金黄色葡萄球菌ATCC 6538P和金黄色葡萄球菌810生物膜的影响。土耳其的植物材料包括刺柏Juniperus communis L. var. communis、刺柏J. communis L. var. saxatilis Pall.、地中海刺柏Juniperus drupacea Labill.、刺柏Juniperus oxycedrus L. ssp. oxycedrus、刺柏J. oxycedrus L. ssp. macrocarpa (Sibth. & Sm.) Ball。通过薄层色谱法对刺柏属植物提取物进行了初步的植物化学分析。使用最低抑菌浓度（MIC）和最低杀菌浓度（MBC）评估抗菌活性。通过生物量OD和CFU计数法对提取物对生物膜形成和预先形成的生物膜的影响进行了定量。植物化学筛选显示存在多酚、香豆素、木脂素、甾体、生物碱和萜类化合物。对于这两种菌株，所有提取物的MIC范围为4.88-78.12μg mL(-1)。对于金黄色葡萄球菌ATCC 6538P，提取物的亚抑菌浓度（0.5 MIC）对浮游生长和粘附细胞的影响最小，而对生物膜形成的影响更大。不同的是，对于金黄色葡萄球菌810，它们对生物膜形成仅显示出相当低的效力。2 MIC的提取物对金黄色葡萄球菌ATCC 6538P的预先形成的生物膜表现出良好的活性。
