Tatskaia V N, Nozdrin V I, Kvachev V G
Biull Eksp Biol Med. 1989 Jul;108(7):93-5.
The 0.001-0.005% retinoic acid injection into the growth cultural medium of prime and continue cell cultures 12-24h before inoculation considerably raised the cell sensitivity to animal entero- and coronaviruses. The entero- and coronaviruses concentrations in cultural medium increased by 10(1.58) and 10(1.68)TCID 50/1.0 respectively. The optimized parameters of the cell culture processing for the enteropathogenic viruses reproduction improvement are proposed.
在接种前12 - 24小时，向原代和传代细胞培养物的生长培养基中注射0.001 - 0.005%的视黄酸，可显著提高细胞对动物肠道病毒和冠状病毒的敏感性。培养基中肠道病毒和冠状病毒的浓度分别增加了10(1.58)和10(1.68)TCID 50/1.0。提出了优化细胞培养处理参数以提高肠道致病病毒繁殖的方法。
