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采用好氧颗粒污泥序批式生物反应器修复药品和个人护理产品,并采用 Solexa 测序技术分析微生物群落特征。

Remediation of pharmaceuticals and personal care products using an aerobic granular sludge sequencing bioreactor and microbial community profiling using Solexa sequencing technology analysis.

机构信息

State Key Laboratory of Urban Water Resource and Environment, School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China; College of Petrochemical Technology, Lanzhou University of Technology, Lanzhou 730050, China.

State Key Laboratory of Urban Water Resource and Environment, School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.

出版信息

Bioresour Technol. 2015 Mar;179:104-112. doi: 10.1016/j.biortech.2014.12.002. Epub 2014 Dec 11.

Abstract

Recently, a new type of organic pollution derived from pharmaceuticals and personal care products (PPCPs) is gradually on the rise. Wastewater treatment to remove PPCPs was investigated using an aerobic granular sludge sequencing bioreactor (GSBR). After optimization of influent organic load, hydraulic shear stress, sludge settling time, etc., aerobic granular sludge was analyzed for its physiological and biochemical characteristics and tested for its efficacy to remove PPCPs wastewater. The granular sludge effectively removed some but not all of the PPCPs tested; removal correlated with the microbial profiles in the granules, as assessed using Solexa sequencing technology. Sequencing revealed the presence of five phylogenetic groups: Proteobacteria, Bacteroidetes, Betaproteobacteria, an unclassified genus, and Zoogloea. The results demonstrated changes in the microbial profiles with time in response to the presence of PPCPs. The effects of PPCPs on microbial communities in granular sludge process are discussed.

摘要

最近,一种新型的有机污染物逐渐兴起,其来源于药品和个人护理产品(PPCPs)。采用好氧颗粒污泥序批式生物反应器(GSBR)对去除 PPCPs 的废水处理进行了研究。在优化了进水有机负荷、水力剪切应力、污泥沉降时间等条件后,对好氧颗粒污泥的生理生化特性进行了分析,并对其去除 PPCPs 废水的效果进行了测试。颗粒污泥有效去除了部分而非全部测试的 PPCPs;去除效果与使用 Solexa 测序技术评估的颗粒中微生物的分布情况相关。测序结果表明,存在着五个系统发育群:变形菌门、拟杆菌门、β变形菌门、一个未分类属和动胶菌属。结果表明,随着时间的推移,微生物的分布情况会发生变化,以响应 PPCPs 的存在。本文讨论了 PPCPs 对颗粒污泥工艺中微生物群落的影响。

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