Xu K
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1989 Jun;11(3):195-9.
The MNNG transformed Syrian hamster lung fibroblast cell line (BHLB4) established in our laboratory was treated with 2.0 mmol/L butyric acid. We obtained a butyric acid-dependent partially reversed cell line (ButB4) similar to normal cells in morphology, membrane properties, proliferation rate, colony-forming ability on solid agar culture, etc. However, when transplanted into nude mice, this cell line's tumorigenicity remained intact. Detection of cAMP levels in the cytoplasm showed much higher levels in ButB4 cells than in transformed cells. Butyric acid activity is evidently associated with changes of cAMP. In addition to phenotypical analysis, the karyotypes of ButB4 and BHLB4 were analyzed in detail. It was found that the disomy rate of C15 was significantly increased in ButB4 cells (73%) as compared with BHLB4 cells (33%). These results show that butyric acid not only induces phenotypic reversion in BHLB4 cells directly, but can also selectively kill C15 monosomy cell clones, allowing rapid growth of C15 disomy cell clones. Butyric acid may also influence maintenance of the reversion phenotype.
用2.0 mmol/L丁酸处理我们实验室建立的MNNG转化的叙利亚仓鼠肺成纤维细胞系（BHLB4）。我们获得了一种丁酸依赖性部分逆转细胞系（ButB4），其在形态、膜特性、增殖率、固体琼脂培养上的集落形成能力等方面类似于正常细胞。然而，当将该细胞系接种到裸鼠体内时，其致瘤性仍然存在。对细胞质中cAMP水平的检测显示，ButB4细胞中的cAMP水平比转化细胞中的高得多。丁酸活性显然与cAMP的变化有关。除了表型分析外，还详细分析了ButB4和BHLB4的核型。结果发现，与BHLB4细胞（33%）相比，ButB4细胞中C15的二体率显著增加（73%）。这些结果表明，丁酸不仅能直接诱导BHLB4细胞的表型逆转，还能选择性地杀死C15单体细胞克隆，使C15二体细胞克隆快速生长。丁酸也可能影响逆转表型的维持。
