Xu K, Li S D, Xu D X
Shi Yan Sheng Wu Xue Bao. 1989 Mar;22(1):67-73.
In this paper, Fn was analysed qualitatively and quantitatively in three Syrian hamster cell lines, ie, nontransformed baby hamster lung fibroblasts cell line (BHL), a transformed cell line (BHLB4) and a butyric acid-induced phenotypically reversed cell line (ButB4) respectively. Fn was visualized on cell surface by means of indirect immunofluorescence technique. Immunofluorescence of Fn on the surface of BHL was bright with a stripe-like distribution, while that on the surface of BHLB4 was very dim or dispersed. On ButB4 cell surface, the intensity and distribution of immunofluorescence was similar to that on BHLB4 cells. Fn was isolated by affinity chromatography from the cell surface of the three cell lines. Its molecular weight was 250 kDa on SDS-polyacrylamide electrophoresis. The quantity of Fn isolated from surface of ButB4 was a bit lower than that from BHL, but was much higher than that from BHLB4. The result offers us a useful criterion for transformation and reverse transformation.
本文分别对三种叙利亚仓鼠细胞系，即未转化的幼仓鼠肺成纤维细胞系（BHL）、转化细胞系（BHLB4）和丁酸诱导的表型逆转细胞系（ButB4）中的纤连蛋白（Fn）进行了定性和定量分析。通过间接免疫荧光技术在细胞表面观察Fn。BHL细胞表面Fn的免疫荧光明亮，呈条纹状分布，而BHLB4细胞表面的免疫荧光非常暗淡或呈弥散状。在ButB4细胞表面，免疫荧光的强度和分布与BHLB4细胞相似。通过亲和层析从三种细胞系的细胞表面分离出Fn。在SDS-聚丙烯酰胺电泳上其分子量为250 kDa。从ButB4表面分离出的Fn量略低于从BHL分离出的量，但远高于从BHLB4分离出的量。该结果为转化和逆转转化提供了一个有用的标准。
