a Departamento de Química Orgânica e Inorgânica , Universidade Federal do Ceará , Fortaleza , CE , Brazil.
Nat Prod Res. 2015;29(16):1545-50. doi: 10.1080/14786419.2014.987772. Epub 2014 Dec 23.
A fungal strain of Aspergillus sp. (BRF 030) was isolated from the sediments collected in the northeast coast of Brazil, and the cytotoxic activity of its secondary metabolites was investigated against HCT-116 tumour cell line. The cytotoxicity-guided fractionation of the extracts from this fungus cultured in potato-dextrose-sea water for 14 days at room temperature yielded the hetero-spirocyclic γ-lactams pseurotin A (1), pseurotin D (2) and pseurotin FD-838 (7), the alkaloids fumitremorgin C (5), 12,13-dihydroxy fumitremorgin C (6), methylsulochrin (4) and bis(dethio)bis(methylthio)gliotoxin (3). Among them, fumitremorgin C (5) and 12,13-dihydroxy fumitremorgin C (6) were the most active. The cytotoxic activities of the extracts from Aspergillus sp. grown from 7 to 28 days were investigated, and they were associated with the kinetic production of the compounds. The most active extracts (14 and 21 days) were those with the highest relative concentrations of the compounds fumitremorgin C (5) and 12,13-dihydroxy fumitremorgin C (6).
从巴西东北海岸采集的沉积物中分离到一株曲霉属真菌（BRF 030），研究了其次级代谢产物对 HCT-116 肿瘤细胞系的细胞毒性。从该真菌在马铃薯-葡萄糖-海水培养基中于室温下培养 14 天的提取物中进行细胞毒性导向分离，得到了杂螺环 γ-内酰胺 pseurotin A（1）、pseurotin D（2）和 pseurotin FD-838（7）、生物碱 fumitremorgin C（5）、12,13-二羟基 fumitremorgin C（6）、methylsulochrin（4）和双（脱硫）双（甲基硫代）gliotoxin（3）。其中，fumitremorgin C（5）和 12,13-二羟基 fumitremorgin C（6）活性最强。研究了 Aspergillus sp.从第 7 天到第 28 天生长的提取物的细胞毒性，它们与化合物的动力学产生有关。最活跃的提取物（第 14 天和第 21 天）是那些具有相对较高浓度的化合物 fumitremorgin C（5）和 12,13-二羟基 fumitremorgin C（6）的提取物。
