Salvati F, Teodori L, Gagliardi L, Signora M, Aquilini M, Storniello G
8th Division of Pneumology, C. Forlanini Hospital, Rome, Italy.
Chest. 1989 Nov;96(5):1092-8. doi: 10.1378/chest.96.5.1092.
To investigate the prognostic implications of DNA flow cytometry in human lung tumors, we analyzed specimens from patients with neoplastic and non-neoplastic lung disease. Most non-neoplastic and normal (taken at the resection border) lung samples yielded a single cell population with diploid DNA content (only two normal lung specimens from two cancer patients had aneuploid DNA content). At least one aneuploid cell subpopulation was seen in 91 percent of NSCLC and 50 percent on SCLC. To show intratumor heterogeneity, multiple-site sampling was done whenever possible in both primary tumor and metastatic sites, revealing a high incidence of multiclonality (50 percent). Although diploid tumors were rare, they associated with a higher survival rate than aneuploid monoclonal and multiclonal tumors with hypoploid and/or hypertetraploid clones, which had the lowest survival. Cellular DNA content analysis in patients with lung tumors may be useful in prognostic evaluation.
为了研究DNA流式细胞术在人类肺肿瘤中的预后意义，我们分析了患有肿瘤性和非肿瘤性肺病患者的标本。大多数非肿瘤性和正常（取自切除边缘）肺样本产生具有二倍体DNA含量的单细胞群体（仅两名癌症患者的两个正常肺标本具有非整倍体DNA含量）。在91%的非小细胞肺癌和50%的小细胞肺癌中可见至少一个非整倍体细胞亚群。为了显示肿瘤内异质性，在原发性肿瘤和转移部位尽可能多地进行多点采样，结果显示多克隆性的发生率很高（50%）。虽然二倍体肿瘤很少见，但与具有亚二倍体和/或超四倍体克隆的非整倍体单克隆和多克隆肿瘤相比，它们的生存率更高，而后者的生存率最低。肺肿瘤患者的细胞DNA含量分析可能有助于预后评估。
