Salvati F, Teodori L, Trinca M L, Pasquali-Lasagni R, Göhde W
Division of Pneumology, Forlanini Hospital, Rome, Italy.
J Cancer Res Clin Oncol. 1994;120(4):233-9. doi: 10.1007/BF01372562.
Cells from a group of 185 patients suffering from malignant tumours (160 non-small-cell lung carcinoma, 13 small-cell lung carcinoma, and 12 non-epithelial tumours) and 6 with benign lung tumours were studied by flow cytometry in order to detect the prognostic value of DNA content. A total of 144 (90%) non-small-cell lung carcinomas (NSCLC) and 8 (62%) small-cell lung carcinomas (SCLC) exhibited aneuploidy. Furthermore 52% (83 patients) NSCLC, 24% (3 patients) SCLC and 50% (6 patients) non-epithelial tumours demonstrated multiclonality. Benign cases showed diploid DNA content. For actuarial survival analysis using the Bergesson and Gage method and the Greenwood variance, 142 patients were selected. Statistical comparisons were made by the use of the t-test for unpaired data between fixed times. No correlation was observed between ploidy and stage, histological grading or treatment modality. A statistically significantly better survival was observed after 12, 18 and 24 months of follow-up for diploid and monoclonal (with the exclusion of hypo- and hypertetraploid) patients. Thus, flow-cytometric DNA analysis may be useful in prognostic assessment of human lung tumours.
为检测DNA含量的预后价值，对185例患有恶性肿瘤（160例非小细胞肺癌、13例小细胞肺癌和12例非上皮性肿瘤）的患者及6例患有良性肺肿瘤的患者的细胞进行了流式细胞术研究。共有144例（90%）非小细胞肺癌（NSCLC）和8例（62%）小细胞肺癌（SCLC）表现为非整倍体。此外，52%（83例）NSCLC、24%（3例）SCLC和50%（6例）非上皮性肿瘤表现出多克隆性。良性病例显示二倍体DNA含量。采用Bergesson和Gage方法及Greenwood方差进行精算生存分析，选择了142例患者。通过对固定时间之间的非配对数据使用t检验进行统计比较。未观察到倍性与分期、组织学分级或治疗方式之间的相关性。在随访12、18和24个月后，观察到二倍体和单克隆（排除亚四倍体和超四倍体）患者的生存率在统计学上显著更高。因此，流式细胞术DNA分析可能有助于人类肺肿瘤的预后评估。
