Zhou Min, Mao Lijuan, Wang Ying, Wang Qian, Yang Zhiyun, Li Shurong, Li Ling
Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department, National Key Discipline, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Department of Radiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
J Stroke Cerebrovasc Dis. 2015 Mar;24(3):530-6. doi: 10.1016/j.jstrokecerebrovasdis.2014.09.038. Epub 2014 Dec 19.
The aims of this study were to determine whether arterial hypertension could affect the venous system of brain and to find out the consequent pathologic changes of cerebral veins.
Thirty male Sprague-Dawley rats were divided into 2 groups: a sham-clipped group and a stroke-prone renovascular hypertensive rat group. A 2-kidney 2-clip rat model was used to induce renovascular hypertension in the hypertensive group. Systolic blood pressure was measured by tail cuff once each week. Susceptibility-weighted imaging (SWI) was performed at 12, 16, and 20 weeks after surgery. All the rats were sacrificed after the SWI examination at 20 weeks after surgery. The brains were extracted and embedded in paraffin for histologic examination. Masson trichrome staining was performed to identify venous collagenosis.
The sham group demonstrated less prominence of cerebral veins compared with hypertensive groups (P < .01); the hypertensive group showed significant venous collagenosis in cerebral venous walls compared with the sham group (P < .01).
The increased visibility of cerebral veins on SWI as a sign of venous hypertension and the thickened cerebral venous walls (venous collagenosis), which may play a role in cerebral ischemia and/or infarction, are both consequences of long-term hypertension in hypertensive rats.
本研究的目的是确定动脉高血压是否会影响脑静脉系统，并找出脑静脉随之发生的病理变化。
30只雄性Sprague-Dawley大鼠被分为2组：假手术夹闭组和易卒中型肾血管性高血压大鼠组。采用双肾双夹大鼠模型在高血压组诱导肾血管性高血压。每周用尾套法测量收缩压。在术后12、16和20周进行磁敏感加权成像（SWI）。术后20周进行SWI检查后处死所有大鼠。取出大脑并嵌入石蜡进行组织学检查。采用马松三色染色法鉴定静脉胶原化。
与高血压组相比，假手术组脑静脉不那么突出（P <.01）；与假手术组相比，高血压组脑静脉壁出现明显的静脉胶原化（P <.01）。
SWI上脑静脉的可视性增加作为静脉高压的标志，以及脑静脉壁增厚（静脉胶原化），这可能在脑缺血和/或梗死中起作用，都是高血压大鼠长期高血压的后果。
