Zeng J, Zhang Y, Mo J, Su Z, Huang R
Department of Neurology, First Affiliated Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou, People's Republic of China.
Stroke. 1998 Aug;29(8):1708-13; discussion 1713-4. doi: 10.1161/01.str.29.8.1708.
The cerebrovascular lesions in stroke-prone spontaneously hypertensive rats are not only dependent on high blood pressure but partly related to pressure-independent genetic factors. The aim of the present study was to observe whether spontaneous stroke occurred in renovascular hypertensive rats without a genetic deficiency.
The 1-kidney, 1 clip (1k1c); 2-kidney, 1 clip (2k1c); and 2-kidney, 2 clip (2k2c) methods were used to induce hypertension in male Sprague-Dawley rats with a ring-shaped silver clip. Sham-operated rats were used as controls. Blood pressure and neurological symptoms were observed in the rats without any artificial inducement. Brain sections stained with hematoxylin-eosin and phosphotungstic acid-hematoxylin were examined under a microscope to determine stroke foci.
The attack rate of stable hypertension was 100% (55/55) in the 2k2c group, which was significantly higher than that in the 1k1c (23/30, 76.7%) and 2k1c (21/30, 70%) groups (P<0.01). None of the rats in the 2k2c group died of acute renal failure or suffered from diffuse cerebral lesions postoperatively. Forty weeks after renal artery constriction, the incidence of spontaneous stroke in the 2k2c group was 61.8% (34/55), which was significant higher than that in the 1k1c (7/30, 23.3%) and 2k1c (5/30,16.7%) groups (P<0.01). Stroke foci were not observed in normotensive controls.
We conclude that 2k2c renovascular hypertensive rats with proper renal artery constriction can be used as stroke-prone renovascular hypertensive rats independent of a genetic deficiency.
易中风自发性高血压大鼠的脑血管病变不仅取决于高血压，还部分与压力非依赖性遗传因素有关。本研究的目的是观察无遗传缺陷的肾血管性高血压大鼠是否会发生自发性中风。
采用单肾单夹（1k1c）、双肾单夹（2k1c）和双肾双夹（2k2c）方法，用环形银夹诱导雄性Sprague-Dawley大鼠高血压。假手术大鼠作为对照。在无任何人工诱导的情况下观察大鼠的血压和神经症状。对苏木精-伊红和磷钨酸-苏木精染色的脑切片进行显微镜检查以确定中风病灶。
2k2c组稳定高血压的发生率为100%（55/55），显著高于1k1c组（23/30，76.7%）和2k1c组（21/30，70%）（P<0.01）。2k2c组大鼠术后无一死于急性肾衰竭或患有弥漫性脑病变。肾动脉缩窄40周后，2k2c组自发性中风的发生率为61.8%（34/55），显著高于1k1c组（7/30，23.3%）和2k1c组（5/30，16.7%）（P<0.01）。正常血压对照组未观察到中风病灶。
我们得出结论，适当肾动脉缩窄的2k2c肾血管性高血压大鼠可作为无遗传缺陷的易中风肾血管性高血压大鼠。
