Sun Zongtao, He Yuqing, Li Junmin, Wang Xu, Chen Jianping
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
Plant Cell Physiol. 2015 Apr;56(4):688-99. doi: 10.1093/pcp/pcu213. Epub 2014 Dec 21.
MicroRNAs (miRNAs) are small, non-coding RNAs which typically function by guiding cleavage of target mRNAs. They play important roles in development, abiotic stress and responses to pathogens. Four small RNA libraries and four degradome libraries were constructed from the leaves and roots of healthy rice and plants infected with Rice black streaked dwarf virus (RBSDV). Analysis of the deep sequencing results showed that the expression patterns of 14 miRNAs in leaves and 16 miRNAs in roots changed significantly in response to RBSDV infection. Some responses were similar in roots and leaves, but many miRNAs responded differently in different tissues. The results were confirmed for selected miRNAs by quantitative real-time PCR. By using degradome sequencing, a total of 104 target transcripts for 17 conserved and 16 non-conserved miRNAs were shown to be responsive to RBSDV infection. Fifteen novel miRNAs were also identified by small RNA and degradome sequencing. The results provide new insights into the regulatory networks of miRNAs and their targets in different plant tissues in response to virus infection.
微小RNA（miRNA）是一类小的非编码RNA，通常通过引导靶标mRNA的切割发挥作用。它们在发育、非生物胁迫和对病原体的反应中发挥重要作用。从健康水稻以及感染水稻黑条矮缩病毒（RBSDV）的植株的叶片和根部构建了四个小RNA文库和四个降解组文库。深度测序结果分析表明，响应RBSDV感染，叶片中14个miRNA和根部中16个miRNA的表达模式发生了显著变化。一些反应在根和叶中相似，但许多miRNA在不同组织中的反应不同。通过定量实时PCR对选定的miRNA进行了结果验证。通过降解组测序，发现17个保守和16个非保守miRNA的总共104个靶标转录本对RBSDV感染有反应。通过小RNA和降解组测序还鉴定出15个新的miRNA。这些结果为miRNA及其靶标在不同植物组织中响应病毒感染的调控网络提供了新的见解。
