State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Viruses. 2021 Apr 25;13(5):752. doi: 10.3390/v13050752.
The RAV family is part of the B3 superfamily and is one of the most abundant transcription factor families in plants. Members have highly conserved B3 or AP2 DNA binding domains. Although the family genes of several species have been systematically identified from genome-wide studies, there has been no comprehensive study to identify rice family genes. Here, we identified 15 genes of the RAV family in the rice genome and analyzed their phylogenetic relationships, gene structure, conserved domains, and chromosomal distribution. Based on domain similarity and phylogenetic topology, rice RAV transcription factors were phylogenetically clustered into four groups. qRT-PCR analyses showed that expression of these genes was significantly up-regulated or down-regulated by plant hormone treatments, including BL, NAA, IAA, MeJA, and SA. Most of the rice genes were dramatically down-regulated in response to rice stripe virus (RSV) and mostly up-regulated in response to Southern rice black-streaked dwarf virus (SRBSDV). These results suggest that the rice genes are involved in diverse signaling pathways and in varied responses to virus infection.
RAV 家族是 B3 超家族的一部分，是植物中最丰富的转录因子家族之一。成员具有高度保守的 B3 或 AP2 DNA 结合结构域。尽管已经从全基因组研究中系统地鉴定了几个物种的 家族基因，但还没有对水稻 家族基因进行全面研究。在这里，我们从水稻基因组中鉴定了 15 个 RAV 家族基因，并分析了它们的系统发育关系、基因结构、保守结构域和染色体分布。基于结构域相似性和系统发育拓扑结构，水稻 RAV 转录因子被系统聚类为四个亚组。qRT-PCR 分析表明，这些基因的表达受到植物激素处理的显著上调或下调，包括 BL、NAA、IAA、MeJA 和 SA。大多数水稻 基因对水稻条纹病毒 (RSV) 的反应明显下调，而对南方水稻黑条矮缩病毒 (SRBSDV) 的反应大多上调。这些结果表明，水稻 基因参与多种信号通路，并对病毒感染有不同的反应。
