Józsa L, Kvist M, Lehto M, Réffy A, Vieno T
Department of Morphology, National Institute of Traumatology, Budapest, Hungary.
Gegenbaurs Morphol Jahrb. 1989;135(3):519-28.
Inclusion bodies containing glycogen-enzymes were found in 30 to 60% of type 2 fibres of tenotomized calf muscles (m. gastrocnemius, m. soleus, m. plantaris) in rats, using histochemical reactions. The bodies appeared within 1 week after the tenotomy and were localized both in the central and the subsarcolemmal regions and rarely extruded into the extracellular space. These aggregates are 3 to 15 microns in length and 2 to 11 microns in diameter. In addition to glycogen, these bodies also contained various enzymes of the glycogen metabolism such as phosphorylase, a branching enzyme, and glucose-6-phosphatase, but showed no NADH-reductase, lactate dehydrogenase, or myofibrillar ATP-ase activity. The results indicate that glycogen-enzymes containing bodies are a degenerative phenomenon, which occurs only in type 2 fibres of the tenotomized muscles.
运用组织化学反应，在大鼠跟腱切断的小腿肌肉（腓肠肌、比目鱼肌、跖肌）的30%至60%的Ⅱ型纤维中发现了含有糖原酶的包涵体。这些包涵体在跟腱切断术后1周内出现，位于中央区和肌膜下区域，很少挤出到细胞外间隙。这些聚集体长度为3至15微米，直径为2至11微米。除糖原外，这些包涵体还含有糖原代谢的各种酶，如磷酸化酶、分支酶和葡萄糖-6-磷酸酶，但未显示NADH还原酶、乳酸脱氢酶或肌原纤维ATP酶活性。结果表明，含有糖原酶的包涵体是一种退行性现象，仅发生在跟腱切断肌肉的Ⅱ型纤维中。
