Ricevuti G, Mazzone A, Monaia C, Fratino P, Degiulio R, Dell'Acqua R, Leonardi G, Jucci A, Sacchi S
Department of Internal Medicine and Therapeutics, University of Pavia, Italy.
Inflammation. 1989 Oct;13(5):507-27. doi: 10.1007/BF00916758.
The goal of this work was to evaluate the effect of helium-neon (HeNe) laser irradiation on immunocompetent cells. We used the in vivo skin window method and in vitro granulocyte function tests. The study of cellular migration showed a marked decrease in vitro and in vivo in a dose-independent manner. Superoxide release was not modified by laser irradiation. The granulocyte's aggregation, when using PHA and PMA, presented a reduction that was statistically very significant, not as a subordinate dose. An increase of the release of ATP was demonstrated only at 4 joules and precedes granulocyte aggregation. When using Ca2+ ionophore A23187 as stimulus, laser irradiation at 1, 2 or 4J did not show any modification of granulocyte aggregation. The monoclonal antibody 60.1, which identifies a membrane antigen fundamental for aggregation and chemotaxis, is expressed in normal amounts on granulocyte membranes both before and after irradiation with a HeNe laser. In fact, Laser irradiation preferentially attacks the area of the cellular centrosome that determines a modification of cellular morphology. The electron microscope and immunofluorescence study with a monoclonal antibody have pointed out a disorganization of the microtubules. The alteration of some of the granulocyte functions is correlated to the damage in the centrioles. The granulocyte mitochondrial system and surface membrane remain intact, and this explains the normal production and release of free radicals. Further experiments are necessary to evaluate the clinical application of lasers in various diseases with immunophagocytic pathogenesis.
这项工作的目的是评估氦氖（HeNe）激光照射对免疫活性细胞的影响。我们采用了体内皮肤窗法和体外粒细胞功能测试。细胞迁移研究表明，在体外和体内均呈剂量依赖性显著下降。激光照射未改变超氧化物的释放。使用PHA和PMA时，粒细胞的聚集呈现出统计学上非常显著的降低，并非呈从属剂量关系。仅在4焦耳时证明ATP释放增加，且发生在粒细胞聚集之前。当使用Ca2+离子载体A23187作为刺激物时，1、2或4焦耳的激光照射未显示粒细胞聚集有任何改变。识别聚集和趋化作用所必需的膜抗原的单克隆抗体60.1，在氦氖激光照射前后，粒细胞膜上的表达量均正常。事实上，激光照射优先攻击决定细胞形态改变的细胞中心体区域。电子显微镜和单克隆抗体免疫荧光研究指出微管排列紊乱。粒细胞某些功能的改变与中心粒损伤有关。粒细胞线粒体系统和表面膜保持完整，这解释了自由基的正常产生和释放。有必要进行进一步实验，以评估激光在各种具有免疫吞噬发病机制的疾病中的临床应用。
