Lawton J W, Robinson J P, Till G O
Department of Pathology, University of Hong Kong.
Immunopharmacology. 1989 Sep-Oct;18(2):97-105. doi: 10.1016/0162-3109(89)90062-3.
Three commercially available preparations of human immunoglobulin for intravenous use (IVIgG), namely Gamimune N. Sandoglobulin and Intraglobin F, were tested for their ability to modulate human neutrophil function in vitro. IVIgG consistently stimulated the neutrophil respiratory burst at concentrations of 0.5 to 1 mg/ml, concentrations readily achieved in vivo by moderate-dose therapy. Superoxide (O2-) release was increased by 3.5-4.5 nmol per 5 x 10(5) cells at these concentrations of IVIgG, and H2O2 production increased in a dose-dependent fashion up to 8 mg/ml IVIgG. Luminol-dependent chemiluminescence (CL) was also directly stimulated by IVIgG. In addition, the effects of both soluble and particulate stimulators (N-formyl-methionyl-leucyl-phenylalanine, phorbol myristate acetate and opsonized zymosan) on the neutrophil respiratory burst were enhanced by IVIgG. In a filter assay of neutrophil migration, using a modified Boyden chamber, no consistent effect on neutrophil locomotion or chemotaxis could be demonstrated. The effect of IVIgG on neutrophil metabolism may contribute to its beneficial therapeutic effect in severe, life-threatening infections.
对三种市售的静脉用人免疫球蛋白制剂（IVIgG），即Gamimune N、Sandoglobulin和Intraglobin F，进行了体外调节人中性粒细胞功能能力的测试。IVIgG在浓度为0.5至1mg/ml时持续刺激中性粒细胞呼吸爆发，这种浓度通过中等剂量治疗在体内很容易达到。在这些IVIgG浓度下，每5×10⁵个细胞的超氧化物（O₂⁻）释放量增加3.5 - 4.5nmol，并且H₂O₂生成量以剂量依赖方式增加，直至IVIgG浓度达到8mg/ml。IVIgG还直接刺激鲁米诺依赖性化学发光（CL）。此外，IVIgG增强了可溶性和颗粒性刺激物（N-甲酰甲硫氨酰亮氨酰苯丙氨酸、佛波酯肉豆蔻酸酯和调理酵母聚糖）对中性粒细胞呼吸爆发的影响。在使用改良博伊登小室的中性粒细胞迁移滤膜试验中，未证明对中性粒细胞运动或趋化性有一致影响。IVIgG对中性粒细胞代谢的作用可能有助于其在严重的、危及生命的感染中产生有益的治疗效果。
