Cavic Milena, Krivokuca Ana, Spasic Jelena, Brotto Ksenija, Malisic Emina, Radosavljevic Davorin, Radulovic Sinisa, Jankovic Radmila
Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Belgrade, Serbia.
J BUON. 2014 Oct-Dec;19(4):1024-8.
Methylenetetrahydrofolate reductase (MTHFR) and thymidylate synthetase (TYMS) are suggested as risk factors for lung cancer. The purpose of this study was to analyze the association of MTHFR C677T polymorphism and variable number tandem repeat 2R/3R and single nucleotide polymorphism G>C in the 3R allele of the TYMS gene with lung adenocarcinoma.
A case-control study including lung adenocarcinoma patients and healthy subjects was performed. Restriction fragment length polymorphism analysis was used for genotyping. Descriptive analyses included genotype and allelic frequencies; the odds ratio and 95% confidence interval were calculated as an estimate of relative risk. Significance was set at p<0.05.
A significant difference in CC vs TT+CT MTHFR genotype distribution was observed between patients and controls. There was no significant association between the TYMS polymorphisms and the risk of lung adenocarcinoma.
The MTHFR 677T allele is likely to have a protective effect against lung adenocarcinoma development.
亚甲基四氢叶酸还原酶（MTHFR）和胸苷酸合成酶（TYMS）被认为是肺癌的危险因素。本研究的目的是分析MTHFR基因C677T多态性、TYMS基因可变数目串联重复序列2R/3R以及3R等位基因中的单核苷酸多态性G>C与肺腺癌的相关性。
进行了一项包括肺腺癌患者和健康受试者的病例对照研究。采用限制性片段长度多态性分析进行基因分型。描述性分析包括基因型和等位基因频率；计算比值比和95%置信区间作为相对风险的估计值。显著性设定为p<0.05。
患者与对照组之间在MTHFR基因CC与TT+CT基因型分布上存在显著差异。TYMS基因多态性与肺腺癌风险之间无显著相关性。
MTHFR 677T等位基因可能对肺腺癌的发生具有保护作用。
