Farh Mohamed El-Agamy, Kim Yeon-Ju, Van An Hoang, Sukweenadhi Johan, Singh Priyanka, Huq Md Amdadul, Yang Deok-Chun
Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, Yongin, Gyeonggi-do, 449-701, Republic of Korea.
Arch Microbiol. 2015 Apr;197(3):439-47. doi: 10.1007/s00203-014-1075-y. Epub 2014 Dec 24.
Strain DCY85(T) and DCY85-1(T), isolated from rhizosphere of ginseng, were rod-shaped, Gram-reaction-negative, strictly aerobic, catalase positive and oxidase negative. 16S rRNA gene sequence analysis revealed that strain DCY85(T) as well as DCY85-1(T) belonged to the genus Burkholderia and were closely related to Burkholderia fungorum KACC 12023(T) (98.1 and 98.0 % similarity, respectively). The major polar lipids of strain DCY85(T) and DCY85-1(T) were phosphatidylethanolamine, one unidentified aminolipid and two unidentified phospholipids. The major fatty acids of both strains are C16:0, C18:1 ω7c and summed feature 3 (C16:1 ω6c and/or C16:1 ω7c). The predominant isoprenoid quinone of each strain DCY85(T) and DCY85-1(T) was ubiquinone (Q-8) and the G+C content of their genomic DNA was 66.0 and 59.4 mol%, respectively, which fulfill the characteristic range of the genus Burkholderia. The polyamine content of both DCY85(T) and DCY85-1(T) was putrescine. Although both DCY85(T) and DCY85-1(T) have highly similar 16S rRNA and identical RecA and gyrB sequences, they show differences in phenotypic and chemotaxonomic characteristics. DNA-DNA hybridization results proved the consideration of both strains as two different species. Based on the results from our polyphasic characterization, strain DCY85(T) and DCY85-1(T) are considered novel Burkholderia species for which the name Burkholderia ginsengiterrae sp. nov and Burkholderia panaciterrae sp. nov are, respectively, proposed. An emended description of those strains is also proposed. DCY85(T) and DCY85-1(T) showed antagonistic activity against the common root rot pathogen of ginseng, Cylindrocarpon destructans. The proposed type strains are DCY85(T) (KCTC 42054(T) = JCM 19888(T)) and DCY85-1(T) (KCTC 42055(T) = JCM 19889(T)).
从人参根际分离出的菌株DCY85(T)和DCY85-1(T)呈杆状，革兰氏阴性，严格需氧，过氧化氢酶阳性，氧化酶阴性。16S rRNA基因序列分析表明，菌株DCY85(T)和DCY85-1(T)属于伯克霍尔德氏菌属，与真菌伯克霍尔德氏菌KACC 12023(T)密切相关（相似性分别为98.1%和98.0%）。菌株DCY85(T)和DCY85-1(T)的主要极性脂类为磷脂酰乙醇胺、一种未鉴定的氨基脂类和两种未鉴定的磷脂类。两株菌的主要脂肪酸为C16:0、C18:1 ω7c和特征性总和3（C16:1 ω6c和/或C16:1 ω7c）。每个菌株DCY85(T)和DCY85-1(T)的主要类异戊二烯醌为泛醌（Q-8），其基因组DNA的G+C含量分别为66.0和59.4 mol%，符合伯克霍尔德氏菌属的特征范围。DCY85(T)和DCY85-1(T)的多胺含量均为腐胺。尽管DCY85(T)和DCY85-1(T)具有高度相似的16S rRNA以及相同的RecA和gyrB序列，但它们在表型和化学分类特征上存在差异。DNA-DNA杂交结果证明这两株菌应被视为两个不同的物种。基于我们多相分类的结果，菌株DCY85(T)和DCY85-1(T)被认为是伯克霍尔德氏菌属的新物种，分别提议命名为参地伯克霍尔德氏菌（Burkholderia ginsengiterrae sp. nov）和参际伯克霍尔德氏菌（Burkholderia panaciterrae sp. nov）。还提议了对这些菌株的修订描述。DCY85(T)和DCY85-1(T)对人参常见根腐病原菌毁灭柱孢（Cylindrocarpon destructans）表现出拮抗活性。提议的模式菌株为DCY85(T)（KCTC 42054(T)=JCM 19888(T)）和DCY85-1(T)（KCTC 42055(T)=JCM 19889(T)）。
