Department of Oriental Medicinal Biotechnology, Kyung Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do, 17104, Republic of Korea.
Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do, 17104, Republic of Korea.
Arch Microbiol. 2020 Aug;202(6):1341-1347. doi: 10.1007/s00203-020-01840-z. Epub 2020 Mar 9.
A new bacterium, designated DCY113, was isolated from ginseng cultivation soil in Gochang-gun, South Korea, and its taxonomic position identified by the polyphasic approach. 16S rRNA gene sequence analysis determined that this isolate belongs to the genus Paraburkholderia, and was closest to P. dipogonis DL7 (98.6%), P. phytofirmans PsJN (98.5%), P. kirstenboschensis Kb15 (98.4%) and P. aromaticivorans BN (98.1%). Strain DCY113 is Gram-reaction negative, strictly aerobic, rod-shaped, non-motile, and catalase and oxidase positive. The predominant isoprenoid quinone of DCY113 was ubiquinone Q-8. The major cellular fatty acids were C, cyclo-C and the Summed feature 8 (Cω7c and/or Cω6c). The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and an unknown amino lipid (AL1). The G+C content of the genomic DNA was 62.2 mol%. Average nucleotide identity (ANI) between strain DCY113 and the related Paraburkholderia type strains were below the threshold value for species delineation. This low DNA relatedness in combination with phylogenetic and phenotypic tests indicates that strain DCY113 cannot be assigned to any recognized species. Strain DCY113 was also found to have antifungal activity against the pathogenic fungi Cylindrocarpon destructans. In conclusion, this study found DCY113 to be a novel species within the genus Paraburkholderia, for which the name P. panacisoli is proposed. The type strain is DCY113 (= KCTC 52951 = JCM 32098).
一株新的细菌，命名为 DCY113，从韩国高敞郡的人参种植土壤中分离出来，并通过多相方法确定其分类地位。16S rRNA 基因序列分析表明，该分离株属于伯克霍尔德氏菌属，与 P. dipogonis DL7（98.6%）、P. phytofirmans PsJN（98.5%）、P. kirstenboschensis Kb15（98.4%）和 P. aromaticivorans BN（98.1%）最为接近。菌株 DCY113 革兰氏反应阴性，严格需氧，杆状，无动力，过氧化氢酶和氧化酶阳性。DCY113 的主要类异戊二烯醌为泛醌 Q-8。主要的细胞脂肪酸为 C、环-C 和特征脂肪酸 8（Cω7c 和/或 Cω6c）。主要的极性脂为双磷脂酰甘油（DPG）、磷脂酰甘油（PG）、磷脂酰乙醇胺（PE）和一种未知的氨基酸脂（AL1）。基因组 DNA 的 G+C 含量为 62.2 mol%。菌株 DCY113 与相关的伯克霍尔德氏菌模式菌株之间的平均核苷酸同一性（ANI）低于种系划分的阈值。这种低的 DNA 相关性结合进化和表型测试表明，菌株 DCY113 不能被分配到任何已知的种。菌株 DCY113 还被发现对致病真菌 Cylindrocarpon destructans 具有抗真菌活性。总之，本研究发现 DCY113 是伯克霍尔德氏菌属的一个新种，建议命名为 P. panacisoli。模式菌株为 DCY113（=KCTC 52951=JCM 32098）。
