Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
J Microbiol. 2019 Apr;57(4):232-237. doi: 10.1007/s12275-019-8463-6. Epub 2019 Jan 16.
A Gram-stain-negative, strictly aerobic bacterial strain, designated strain S27, was isolated from soil near an artificial pond in South Korea. Cells were non-motile short rods showing oxidase- and catalase-positive activities. Growth of strain S27 was observed at 20-40°C (optimum, 30°C), pH 5.0-7.0 (optimum, pH 6.0), and 0-0.5% (w/v) NaCl (optimum, 0%). Ubiquinone-8 was detected as the sole respiratory quinone and the major fatty acids were C, cyclo-C, and cyclo-Cω8c. The G + C content of the genomic DNA was 62.4 mol%. Phosphatidylglycerol, phosphatidylethanolamine, and an unidentified aminophospholipid were detected as the major polar lipids. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S27 formed a clearly distinct phyletic lineage from closely related Paraburkholderia species within the genus Paraburkholderia. Strain S27 was most closely related to Paraburkholderia rhynchosiae WSM3937, Paraburkholderia ginsengiterrae DCY85, Paraburkholderia fungorum NBRC 102489, and Paraburkholderia graminis C4D1M with 98.8%, 98.4%, 98.4%, and 97.7% 16S rRNA gene sequence similarities, respectively. The DNA-DNA relatedness level between strain S27 and the type strain of P. rhynchosiae was 36.8 ± 2.6%. On the basis of phenotypic, chemotaxonomic and molecular properties, strain S27 represents a novel species of the genus Paraburkholderia, for which the name Paraburkholderia lacunae sp. nov. is proposed. The type strain is S27 (KACC 19714 = JCM 32721).
一株革兰氏阴性、严格需氧的细菌菌株，被命名为 S27 菌株，从韩国人工池塘附近的土壤中分离得到。细胞呈非运动性短杆状，氧化酶和过氧化氢酶呈阳性反应。S27 菌株的生长条件为 20-40°C（最佳温度为 30°C）、pH5.0-7.0（最佳 pH 值为 6.0）和 0-0.5%（w/v）NaCl（最佳为 0%）。细胞中检测到的唯一呼吸醌为泛醌-8，主要脂肪酸为 C、环-C 和环-Cω8c。基因组 DNA 的 G+C 含量为 62.4mol%。鉴定出的主要极性脂类为磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的氨基磷脂。基于 16S rRNA 基因序列的系统发育分析表明，S27 菌株与该属中密切相关的 Paraburkholderia 种形成了一个明显不同的进化枝。S27 菌株与 Paraburkholderia rhynchosiae WSM3937、Paraburkholderia ginsengiterrae DCY85、Paraburkholderia fungorum NBRC 102489 和 Paraburkholderia graminis C4D1M 的 16S rRNA 基因序列相似性最高，分别为 98.8%、98.4%、98.4%和 97.7%。S27 菌株与 Paraburkholderia rhynchosiae 模式菌株的 DNA-DNA 同源性为 36.8±2.6%。基于表型、化学分类和分子特性，菌株 S27 代表 Paraburkholderia 属的一个新种，命名为 Paraburkholderia lacunae sp. nov.。模式菌株为 S27（KACC 19714=JCM 32721）。
