Zhang Lei, Gong Ji T, Zhang Hu Q, Song Quan H, Xu Guang H, Cai Lei, Tang Xiao D, Zhang Hai F, Liu Fang-E, Jia Zhan S, Zhang Hong W
Experiment Teaching Center of Basic Medicine, The Fourth Military Medical University, Xi'an, Shan Xi province, China.
Department of General Surgery, No.406 hospital, Da Lian, Liao Ning Province, China.
J Neurogastroenterol Motil. 2015 Mar 30;21(2):189-99. doi: 10.5056/jnm14119.
BACKGROUND/AIMS: There are increasing evidences for gastrointestinal motility disorder (GIMD) and gastric stress ulcer induced by noise stress. The present study was to investigate the reversed effect of melatonin on GIMD and gastric stress ulcer induced by noise stress and potential mechanism.
Noise stress was induced on rats, and melatonin (15 mg/kg) was administered to rats by intraperitoneal injection. Differences were assessed in gastric residual rate (GRR), small intestine propulsion rate (SPR), Guth injury score, cortisol, gastrointestinal hormones (calcitonin-gene-related peptide and motilin) and oxidative stress markers (superoxide dismutase and malondialde hyde) in blood plasma as well as gastric mucosa homogenate with or without melatonin. The pathological examination of gastric mucosa was also performed.
The GRR and SPR were improved by noise stress compared with control (P < 0.05). The pathological examination and Guth injury score revealed gastric stress ulcer. Moreover, the levels of cortisol, motilin and malondialdehyde in blood plasma and ma-londialdehyde in gastric mucosa homogenate were increased by noise stress (P < 0.05). CGRP and superoxide dismutase activ-ity in both of blood plasma and gastric mucosa homogenate were significantly decreased (P< 0.05). Furthermore, melatonin reversed changes in GRR, SPR, pathological examination, Guth injury score, cortisol, motilin, CGRP, superoxide dismutase activity and malondialdehyde (P < 0.05).
Melatonin is effective in reversing the GIMD and gastric stress ulcer induced by noise stress. The underlying mechanism may be involved in oxidative stress and gastrointestinal hormones.
背景/目的：越来越多的证据表明，噪声应激可导致胃肠动力障碍（GIMD）和胃应激性溃疡。本研究旨在探讨褪黑素对噪声应激诱导的GIMD和胃应激性溃疡的逆转作用及其潜在机制。
对大鼠施加噪声应激，通过腹腔注射给予大鼠褪黑素（15mg/kg）。评估有无褪黑素时血浆及胃黏膜匀浆中胃残留率（GRR）、小肠推进率（SPR）、Guth损伤评分、皮质醇、胃肠激素（降钙素基因相关肽和胃动素）以及氧化应激标志物（超氧化物歧化酶和丙二醛）的差异。同时对胃黏膜进行病理检查。
与对照组相比，噪声应激使GRR和SPR升高（P<0.05）。病理检查和Guth损伤评分显示存在胃应激性溃疡。此外，噪声应激使血浆中皮质醇、胃动素和丙二醛水平以及胃黏膜匀浆中丙二醛水平升高（P<0.05）。血浆和胃黏膜匀浆中的降钙素基因相关肽和超氧化物歧化酶活性均显著降低（P<0.05）。此外，褪黑素逆转了GRR、SPR、病理检查、Guth损伤评分、皮质醇、胃动素、降钙素基因相关肽、超氧化物歧化酶活性和丙二醛的变化（P<0.05）。
褪黑素可有效逆转噪声应激诱导的GIMD和胃应激性溃疡。其潜在机制可能与氧化应激和胃肠激素有关。
