Bruet Vincent, Lieubeau Blandine, Herve Julie, Roussel Anne, Imparato Laëtitia, Desfontis Jean-Claude, Bourdeau Patrick
Unité Dermatologie, Parasitologie, Mycologie, LUNAM, ONIRIS - Nantes-Atlantic National College of Veterinary Medicine, Food Science and Engineering, NANTES, Loire Atlantique, France.
Unité de Pharmacologie Fonctionnelle et de Pathophysiologie Animale, LUNAM, ONIRIS - Nantes-Atlantic National College of Veterinary Medicine, Food Science and Engineering, NANTES, Loire Atlantique, France.
Vet Dermatol. 2015 Jun;26(3):160-4, e33. doi: 10.1111/vde.12191. Epub 2014 Dec 24.
The bone marrow may be involved in human atopic diseases, as shown by the release of CD34+ cells into the peripheral blood.
HYPOTHESIS/OBJECTIVES: The aim was to determine the numbers of CD34+ cells in atopic dogs.
The following three groups of dogs were studied: 27 dogs with nonfood-induced atopic dermatitis (NFICAD); 16 dogs with nonallergic inflammatory diseases; and 13 healthy control dogs.
Dogs with NFICAD were selected after fulfilment of Favrot's criteria and exclusion of other pruritic dermatoses, including flea infestation and adverse reaction to foods. The Canine Atopic Dermatitis Extent and Severity Index (CADESI)-03 and a Visual Analog Scale (VAS) score for pruritus were used to quantify clinical signs. A phycoerythrin-conjugated anticanine CD34 antibody was used to stain peripheral blood CD34+ cells, and these were enumerated using a flow cytometer. The CD34+ cell counts were compared between groups and tested (in the NFICAD group) for correlation with the severity of clinical signs.
The numbers of peripheral CD34+ cells in dogs with NFICAD (median 1.7) were statistically higher than in dogs with other nonallergic inflammatory diseases (median 1.0; P = 0.01) and healthy control dogs (median 0.9; P = 0.009). In dogs with NFICAD, there was no correlation between CD34+ cell numbers and CADESI-03 scores or owner-assessed pruritus (VAS score).
The results of this study suggest the possible involvement of CD34+ cells in dogs with NFICAD. The role of CD34+ cells in the aetiopathogenesis of canine atopic dermatitis remains to be determined.
骨髓可能参与人类特应性疾病，外周血中CD34+细胞的释放即表明了这一点。
假设/目的：目的是确定特应性犬中CD34+细胞的数量。
研究了以下三组犬：27只患有非食物性特应性皮炎（NFICAD）的犬；16只患有非过敏性炎症性疾病的犬；以及13只健康对照犬。
符合法夫罗标准并排除其他瘙痒性皮肤病（包括跳蚤感染和食物不良反应）后，选择患有NFICAD的犬。使用犬特应性皮炎范围和严重程度指数（CADESI）-03和瘙痒视觉模拟量表（VAS）评分来量化临床症状。使用藻红蛋白偶联的抗犬CD34抗体对外周血CD34+细胞进行染色，并使用流式细胞仪对其进行计数。比较各组之间的CD34+细胞计数，并在NFICAD组中测试其与临床症状严重程度的相关性。
患有NFICAD的犬外周血CD34+细胞数量（中位数1.7）在统计学上高于患有其他非过敏性炎症性疾病的犬（中位数1.0；P = 0.01）和健康对照犬（中位数0.9；P = 0.009）。在患有NFICAD的犬中，CD34+细胞数量与CADESI-03评分或主人评估的瘙痒（VAS评分）之间没有相关性。
本研究结果提示CD34+细胞可能参与了患有NFICAD的犬的发病过程。CD34+细胞在犬特应性皮炎病因发病机制中的作用仍有待确定。
