Singh Mandeep, Kaur Navdeep, Paul Atish Tulsiram
Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India.
Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India ; Department of Pharmacy, Birla Institute of Technology and Science, Pilani, Rajasthan, India.
Anc Sci Life. 2014 Jan;33(3):165-71. doi: 10.4103/0257-7941.144621.
Aśvagandhādyariṣṭa is a polyherbal formulation that is available commercially as an over the counter drug. There are three famous brands that are available in the market. However, there are no comparative reports on the physicochemical, chromatographic, and biological profiles of Aśvagandhādyariṣṭa manufactured by these famous companies.
The present study deals with the physicochemical standardization, high performance thin layer chromatography (HPTLC) profiling, and biological evaluation of Aśvagandhādyariṣṭa.
Aśvagandhādyariṣṭa manufactured by three leading companies were purchased from Jalandhar, Punjab. The physicochemical standardization of the samples was carried out in accordance with the Ayurvedic Pharmacopoeia of India (API). Authentified Eisenia foetida were procured from Ujjwal Ujala Vermiculture Group, Amritsar. The anthelmintic activity, 1,1-diphenyl-2-picrylhydrazyl scavenging, and hydrogen peroxide scavenging ability of Aśvagandhādyariṣṭa was determined.
The data of anthelmintic activity were expressed as mean ± standard error of the mean of six earthworms in each group. The statistical analysis was carried out using one-way analysis of variance, followed by Dunnet t-test. The difference in values at P < 0.05 was considered to be statistically significant.
Most of the physicochemical standardization parameters mentioned as per the API were found to be within limit. HPTLC profiling showed the presence of withanolide D in commercial samples. Out of three commercial brands, ASA-DAB was the most active as compared to the ASA-BDN and ASA-AVP at the concentration of 200 mg/ml for anthelmintic activity against E. foetida. ASA-DAB showed the best antioxidant activity in both the in vitro assay at the concentration of 100 μg/ml.
The ability of this formulation to scavenge free radicals supports its medical claim of antistress formulation. The anthelmintic potential of this formulation helps us conclude that it can also be considered as a general tonic because it provides relief from helminths.
印度人参药酒是一种多草药配方制剂，作为非处方药在市场上有售。市场上有三个著名品牌。然而，关于这些著名公司生产的印度人参药酒的物理化学、色谱和生物学特性，尚无比较报告。
本研究涉及印度人参药酒的物理化学标准化、高效薄层色谱（HPTLC）分析和生物学评价。
从旁遮普邦贾朗达尔购买了三家领先公司生产的印度人参药酒。样品的物理化学标准化按照《印度阿育吠陀药典》（API）进行。从阿姆利则的Ujjwal Ujala Vermiculture Group采购了经鉴定的赤子爱胜蚓。测定了印度人参药酒的驱虫活性、1,1-二苯基-2-苦基肼清除能力和过氧化氢清除能力。
驱虫活性数据表示为每组六条蚯蚓的平均值±平均标准误差。采用单因素方差分析，随后进行Dunnet t检验。P < 0.05时数值的差异被认为具有统计学意义。
发现按照API提到的大多数物理化学标准化参数均在规定范围内。HPTLC分析表明市售样品中存在醉茄内酯D。在三个商业品牌中，在200 mg/ml浓度下，与ASA-BDN和ASA-AVP相比，ASA-DAB对赤子爱胜蚓的驱虫活性最强。在100 μg/ml浓度下，ASA-DAB在两种体外试验中均表现出最佳的抗氧化活性。
该制剂清除自由基的能力支持其作为抗应激制剂的医学宣称。该制剂的驱虫潜力有助于我们得出结论，即它也可被视为一种通用滋补品，因为它能缓解蠕虫感染。
