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QTL 作图揭示了野生豌豆属 Lens ervoides 中真菌病抗性的遗传决定因素。

QTL mapping reveals genetic determinants of fungal disease resistance in the wild lentil species Lens ervoides.

机构信息

Crop Development Centre/Department of Plant Sciences, University of Saskatchewan, Saskatoon, Canada.

Swift Current Research and Development Center, Agriculture and Agri-Food Canada, Swift Current, Canada.

出版信息

Sci Rep. 2017 Jun 12;7(1):3231. doi: 10.1038/s41598-017-03463-9.

DOI:10.1038/s41598-017-03463-9
PMID:28607439
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5468239/
Abstract

Lens ervoides, a wild relative of lentil is an important source of allelic diversity for enhancing the genetic resistance of the cultivated species against economically important fungal diseases, such as anthracnose and Stemphylium blight caused by Colletotrichum lentis and Stemphylium botryosum, respectively. To unravel the genetic control underlying resistance to these fungal diseases, a recombinant inbred line (RIL) population (n = 94, F) originating from a cross between two L. ervoides accessions, L01-827A and IG 72815, was genotyped on the Illumina HiSeq 2500 platform. A total of 289.07 million 100 bp paired-end reads were generated, giving an average 7.53-fold genomic coverage to the RILs and identifying 2,180 high-quality SNPs that assembled in 543 unique haplotypes. Seven linkage groups were resolved among haplotypes, equal to the haploid chromosome number in L. ervoides. The genetic map spanned a cumulative distance of 740.94 cM. Composite interval mapping revealed five QTLs with a significant association with resistance to C. lentis race 0, six QTLs for C. lentis race 1 resistance, and three QTLs for S. botryosum resistance. Taken together, the data obtained in the study reveal that the expression of resistance to fungal diseases in L. ervoides is a result of rearrangement of resistant alleles contributed by both parental accessions.

摘要

野生豌豆属 Lens ervoides 是菜豆种的重要等位基因多样性来源，可增强其对炭疽病和茎点枯病等重要真菌病害的遗传抗性，炭疽病和茎点枯病分别由 Colletotrichum lentis 和 Stemphylium botryosum 引起。为了阐明对这些真菌病的抗性的遗传控制，从两个 Lens ervoides accession（L01-827A 和 IG 72815）的杂交后代中构建了重组自交系（RIL）群体（n=94，F），并在 Illumina HiSeq 2500 平台上进行了基因分型。共生成了 28907 万条 100bp 配对末端reads，平均每个 RIL 的基因组覆盖度为 7.53 倍，共鉴定出 2180 个高质量 SNPs，组装成 543 个独特的单倍型。在单倍型中解析出 7 个连锁群，与 Lens ervoides 的单倍体染色体数相等。遗传图谱共涵盖了 740.94cM 的累积距离。复合区间作图揭示了与 C. lentis 0 小种抗性显著相关的 5 个 QTL、与 C. lentis 1 小种抗性显著相关的 6 个 QTL 和与 S. botryosum 抗性显著相关的 3 个 QTL。综上所述，该研究获得的数据表明，L. ervoides 对真菌病的抗性表达是由两个亲本贡献的抗性等位基因重排的结果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2749/5468239/7032543903dc/41598_2017_3463_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2749/5468239/47cdff50c4bc/41598_2017_3463_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2749/5468239/6fea4270446a/41598_2017_3463_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2749/5468239/e9aac4ba08e6/41598_2017_3463_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2749/5468239/156ec58997ef/41598_2017_3463_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2749/5468239/7032543903dc/41598_2017_3463_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2749/5468239/47cdff50c4bc/41598_2017_3463_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2749/5468239/6fea4270446a/41598_2017_3463_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2749/5468239/e9aac4ba08e6/41598_2017_3463_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2749/5468239/156ec58997ef/41598_2017_3463_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2749/5468239/7032543903dc/41598_2017_3463_Fig5_HTML.jpg

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