蜀葵花朵提取物对雄性大鼠庆大霉素肾毒性缺乏肾保护功效。
Lack of nephroprotective efficacy of althaea officinalis flower extract against gentamicin renal toxicity in male rats.
作者信息
Talebi Ardeshir, Karimi Amirhossein, Ouguerram Khadija, Vahidi-Ataabadi Nasrin, Eshraghi-Jazi Fatemeh, Mansouri Azam, Nematbakhsh Mehdi
机构信息
Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran ; Department of Clinical Pathology, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Nutrition, University of Nantes, Nantes, France.
出版信息
Int J Prev Med. 2014 Nov;5(11):1360-3.
BACKGROUND
Gentamicin (GM) is used as antibiotic for Gram-negative infections, but its administration is limited due to a side-effect of nephrotoxicity. It was attempted to investigate the effect of Althaea officinalis flower extract (AOFE) against nephrotoxicity induced by GM in male rats.
METHODS
30-year-old male Wistar rats were divided into five groups. Group 1 as a negative control group received AOFE 250 mg/kg/day. Groups 2-5 received saline, AOFE 50 mg/kg/day, AOFE 250 mg/kg/day, and AOFE 500 mg/kg/day for 9 days, respectively, and GM (100 mg/kg/day) was added from the 3(rd) day on. At the end of the experiment, blood samples were obtained, animals were sacrificed, and the kidneys were removed immediately.
RESULTS
Gentamicin (in group 2) significantly increased serum levels of blood urea nitrogen and creatinine as well as the pathological damage score (P < 0.05) when compared with group 1. Low dose of AOFE did not decrease the nephrotoxicity induced by GM while the high dose of AOFE aggravated renal toxicity (P < 0.05).
CONCLUSIONS
Although AOFE acts as an antioxidant, at the doses used in the current study did not ameliorate nephrotoxicity induced by GM.
背景
庆大霉素（GM）用作革兰氏阴性菌感染的抗生素，但由于肾毒性副作用，其应用受到限制。本研究旨在探讨药蜀葵花朵提取物（AOFE）对GM诱导的雄性大鼠肾毒性的影响。
方法
将30只雄性Wistar大鼠分为五组。第1组作为阴性对照组，给予AOFE 250 mg/kg/天。第2 - 5组分别给予生理盐水、AOFE 50 mg/kg/天、AOFE 250 mg/kg/天和AOFE 500 mg/kg/天，持续9天，从第3天起添加GM（100 mg/kg/天）。实验结束时，采集血样，处死动物，并立即取出肾脏。
结果
与第1组相比，庆大霉素（第2组）显著提高了血清尿素氮和肌酐水平以及病理损伤评分（P < 0.05）。低剂量的AOFE并未降低GM诱导的肾毒性，而高剂量的AOFE加重了肾毒性（P < 0.05）。
结论
尽管AOFE具有抗氧化作用，但在本研究使用的剂量下，并未改善GM诱导的肾毒性。
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