Analysis and Test Centre of Jiangsu Marine Resources Development Research Institute, Lianyungang 222001, China ; College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, China ; School of Chemistry and Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, China.
College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, China.
J Anal Methods Chem. 2014;2014:964273. doi: 10.1155/2014/964273. Epub 2014 Nov 5.
We proposed a simple and effective method, by coupling ionic liquid-based aqueous two-phase systems (IL-ATPSs) with high performance liquid chromatography (HPLC), for the analysis of determining tartrazine and sunset yellow in food samples. Under the optimized conditions, IL-ATPSs generated an extraction efficiency of 99% for both analytes, which could then be directly analyzed by HPLC without further treatment. Calibration plots were linear in the range of 0.01-50.0 μg/mL for both Ta and SY. The limits of detection were 5.2 ng/mL for Ta and 6.9 ng/mL for SY. This method proves successful for the separation/analysis of tartrazine and sunset yellow in soft drink sample, candy sample, and instant powder drink and leads to consistent results as obtained from the Chinese national standard method.
我们提出了一种简单有效的方法，即将离子液体双水相体系（IL-ATPS）与高效液相色谱（HPLC）相结合，用于分析食品样品中的柠檬黄和日落黄。在优化的条件下，IL-ATPS 对两种分析物的萃取效率均达到 99%，无需进一步处理即可直接用 HPLC 进行分析。两种分析物的校准曲线在 0.01-50.0μg/mL 范围内均呈线性。柠檬黄和日落黄的检出限分别为 5.2ng/mL 和 6.9ng/mL。该方法成功地用于软饮料样品、糖果样品和即溶粉饮料中柠檬黄和日落黄的分离/分析，与中国国家标准方法得到的结果一致。
