Sachan Rekha, Patel Ml, Gaurav Amrita, Gangwar Radheshyam, Sachan Pushpalata
Department of Obstetrics and Gynaecology, King George Medical University, Lucknow, Uttar Pradesh, India.
Department of Medicine, King George Medical University, Lucknow, Uttar Pradesh, India.
Adv Biomed Res. 2014 Nov 29;3:223. doi: 10.4103/2277-9175.145690. eCollection 2014.
Vascular endothelial dysfunction is considered central to the pathogenesis of hypertensive disorders of pregnancy (HDP). Serum level of neutrophil gelatinase-associated lipocalin (NGAL) is closely related to endothelial injury. The aim of this study was to examine the correlation of serum NGAL with disease severity in HDP.
This prospective case-control study was carried out for one year. After informed consent, ethical clearance, total 1,850 pregnant women were screened. Analysis was performed on 142 cases of HDP and 31 healthy controls. Quantitative measurement of serum NGAL levels was done by the enzyme linked immunosorbent assay (ELISA) technique, by using sandwich ELISA kit.
Mean serum NGAL value in patients with oliguria was significantly higher when compared with non-oliguric patients (P < 0.001). Serum NGAL had a positive correlation with systolic blood pressure (r ~ 0.5973), diastolic blood pressure (r ~ 0.6195), blood urea (r ~ 0.4392), serum creatinine (r ~ 0.6112), serum uric acid (r ~ 0.3878). Sensitivity and specificity of serum NGAL using a cut-off value of 545 pg/ml, for the diagnosis of HDP, was 97.89% and 93.55% respectively, using 95% confidence interval.
Between the two groups, we found that serum NGAL had a positive correlation with disease severity and better sensitivity and specificity in the evaluation of HDP.
血管内皮功能障碍被认为是妊娠期高血压疾病（HDP）发病机制的核心。中性粒细胞明胶酶相关脂质运载蛋白（NGAL）的血清水平与内皮损伤密切相关。本研究旨在探讨血清NGAL与HDP疾病严重程度的相关性。
本前瞻性病例对照研究为期一年。在获得知情同意、伦理批准后，共筛查了1850名孕妇。对142例HDP患者和31名健康对照进行了分析。采用酶联免疫吸附测定（ELISA）技术，使用夹心ELISA试剂盒对血清NGAL水平进行定量测定。
少尿患者的血清NGAL平均水平显著高于非少尿患者（P < 0.001）。血清NGAL与收缩压（r ~ 0.5973）、舒张压（r ~ 0.6195）、血尿素（r ~ 0.4392）、血清肌酐（r ~ 0.6112）、血清尿酸（r ~ 0.3878）呈正相关。以545 pg/ml为临界值，血清NGAL诊断HDP的敏感性和特异性分别为97.89%和93.55%（95%置信区间）。
在两组之间，我们发现血清NGAL与疾病严重程度呈正相关，在评估HDP时具有较好的敏感性和特异性。
