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ErbB4作为食管鳞状细胞癌治疗中的潜在分子靶点。

ErbB4 as a potential molecular target in the treatment of esophageal squamous cell cancers.

作者信息

Zhao Ke, Chen Bao-Jun, Chen Zhi-Guo

机构信息

Department of Thoracic Surgery, The Central Hospital of Wuhan, Jiang'an District, Wuhan, Hubei 430014, China.

出版信息

ScientificWorldJournal. 2014;2014:124105. doi: 10.1155/2014/124105. Epub 2014 Nov 4.

DOI:10.1155/2014/124105
PMID:25538945
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4236904/
Abstract

ErbB4 is an important member of ErbB subfamily of tyrosine kinases receptor with overexpression in several tumors; however its biological role in esophageal cancer is poorly understood till date. The main objective of this study was to examine whether miRNA-140-5p could target and control ErbB4 expression at transcriptional level. The ErbB4 expressions in different cell lines were evaluated by western blotting and luciferase assay. Moreover, cell proliferation, apoptosis, and cell invasion studies were investigated using MTT, flow cytometry, and transwell assays. miRNA-140-5p remarkably downregulated the ErbB4 expression in EC9706 and TE-1A cell lines. Furthermore, miRNA-140-5p transfected cell significantly controlled the cell proliferation and enhanced the apoptosis of multiple cells. Additionally, miRNA-140-5p had marked effect on the DNA synthesis and caspase 3/7 activity in comparison to control cells. Specifically, miRNA-140-5p inhibited/repressed the cancer cell invasion and migration in a sign to have important biological role in esophageal carcinomas. Taken together, miRNA-140-5p could act as a potential molecular target in ErbB4 overexpressing ESCC cell lines paving the way for effective esophageal cancer treatment.

摘要

ErbB4是酪氨酸激酶受体ErbB亚家族的重要成员，在多种肿瘤中过表达；然而，迄今为止其在食管癌中的生物学作用仍知之甚少。本研究的主要目的是检测miRNA-140-5p是否能在转录水平靶向并调控ErbB4的表达。通过蛋白质免疫印迹法和荧光素酶测定法评估不同细胞系中ErbB4的表达。此外，使用MTT法、流式细胞术和Transwell实验研究细胞增殖、凋亡及细胞侵袭情况。miRNA-140-5p显著下调了EC9706和TE-1A细胞系中ErbB4的表达。此外，转染miRNA-140-5p的细胞显著抑制了细胞增殖并增强了多种细胞的凋亡。另外，与对照细胞相比，miRNA-140-5p对DNA合成和半胱天冬酶3/7活性有显著影响。具体而言，miRNA-140-5p抑制癌细胞侵袭和迁移，这表明其在食管癌中具有重要的生物学作用。综上所述，miRNA-140-5p可能成为ErbB4过表达的食管鳞状细胞癌（ESCC）细胞系的潜在分子靶点，为食管癌的有效治疗铺平道路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8535/4236904/3fef602aa3f9/TSWJ2014-124105.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8535/4236904/e60c8ed8e553/TSWJ2014-124105.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8535/4236904/f440830dc051/TSWJ2014-124105.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8535/4236904/ec5d973caf13/TSWJ2014-124105.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8535/4236904/05323321e028/TSWJ2014-124105.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8535/4236904/738dd85e4b0f/TSWJ2014-124105.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8535/4236904/3fef602aa3f9/TSWJ2014-124105.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8535/4236904/e60c8ed8e553/TSWJ2014-124105.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8535/4236904/f440830dc051/TSWJ2014-124105.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8535/4236904/ec5d973caf13/TSWJ2014-124105.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8535/4236904/05323321e028/TSWJ2014-124105.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8535/4236904/738dd85e4b0f/TSWJ2014-124105.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8535/4236904/3fef602aa3f9/TSWJ2014-124105.006.jpg

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