Akhtar Tasleem, Sheikh Nadeem, Abbasi Muddasir Hassan
Cell and Molecular Biology Lab, Department of Zoology, University of the Punjab, Q-A Campus, Lahore 54590, Pakistan.
BMC Res Notes. 2014 Dec 23;7:947. doi: 10.1186/1756-0500-7-947.
Nerium oleander has been widely studied for medicinal purposes for variety of maladies. N. oleander has also been reported having noxious effects because of its number of components that may show signs of toxicity by inhibiting plasma lemma Na+, K+-ATPase. The present study was performed to scrutinize the toxic effect of N. oleander leaves extract and its clinical and pathological features in wistar rats.
Hematological analysis showed significant variations in RBCs count (P = 0.01), Hb (P = 0.001), Hct (P = 0.0003), MCV (P = 0.013), lymphocyte count (P = 0.015), neutrophil count (P = 0.003), monocyte count (P = 0.012) and eosinophil count (P = 0.006). Histopathological studies have shown that in T1 group noticeable infiltration of inflammatory cells was found with low level of vascular damage. In T2 group, increased proportion of binucleated and inflammatory cells, hepatic necrosis, widening of sinusoidal spaces and mild level of vascular damage was observed.
Taken together these findings we can conclude that N. oleander leaves extract significantly affects on experimental animals due to its toxicity. Efforts must be exerted to purify different chemical components from extract with no inflammation as this plant is utilized in folk medicine with narrow therapeutic indices.
夹竹桃已被广泛研究用于治疗多种疾病。夹竹桃也有有害影响的报道，因为其多种成分可能通过抑制质膜Na +、K + -ATP酶而显示出毒性迹象。本研究旨在仔细观察夹竹桃叶提取物对Wistar大鼠的毒性作用及其临床和病理特征。
血液学分析显示红细胞计数（P = 0.01）、血红蛋白（P = 0.001）、血细胞比容（P = 0.0003）、平均红细胞体积（P = 0.013）、淋巴细胞计数（P = 0.015）、中性粒细胞计数（P = 0.003）、单核细胞计数（P = 0.012）和嗜酸性粒细胞计数（P = 0.006）有显著变化。组织病理学研究表明，在T1组中发现炎症细胞有明显浸润，血管损伤程度较低。在T2组中，观察到双核细胞和炎症细胞比例增加、肝坏死、窦状隙增宽和轻度血管损伤。
综合这些发现，我们可以得出结论，夹竹桃叶提取物因其毒性对实验动物有显著影响。由于这种植物在民间医学中使用时治疗指数较窄，必须努力从提取物中纯化出无炎症的不同化学成分。
