Strachecka A, Krauze M, Olszewski K, Borsuk G, Paleolog J, Merska M, Chobotow J, Bajda M, Grzywnowicz K
Department of Biological Basis of Animal Production, Faculty of Biology and Animal Breeding, University of Life Sciences in Lublin, Lublin, 20-950, Poland.
Biochemistry (Mosc). 2014 Nov;79(11):1192-201. doi: 10.1134/S0006297914110066.
We examined the influence of caffeine on honeybee lifespan, Nosema resistance, key enzyme activities, metabolic compound concentrations, and total DNA methylation levels. Caffeine slowed age-related metabolic tendencies. Bees that consumed caffeine lived longer and were not infested with Nosema spp. Caffeine-treated workers had higher protein concentrations. The levels increased with aging but they then decreased in older bees. Caffeine increased the activities of antioxidant enzymes (SOD, GPx, CAT, GST), AST, ALT, ALP, neutral proteases, and protease inhibitors, and the concentrations of uric acid, triglycerides, cholesterol, glucose, and Ca2+. Acidic and alkaline protease activities were lower in the bees treated with caffeine. Creatinine and Mg2+ concentrations were higher in the caffeine-treated workers but only up to 14 days of age. Caffeine significantly decreased DNA methylation levels in older bees. The compound could be considered as a natural diet supplement increasing apian resistance to stress factors. Our studies will enhance possibilities of using Apis mellifera as a model organism in gerontological studies.
我们研究了咖啡因对蜜蜂寿命、抗微孢子虫能力、关键酶活性、代谢化合物浓度和总DNA甲基化水平的影响。咖啡因减缓了与年龄相关的代谢趋势。摄入咖啡因的蜜蜂寿命更长，且未感染微孢子虫属。经咖啡因处理的工蜂蛋白质浓度更高。该浓度随年龄增长而升高，但在老年蜜蜂中随后下降。咖啡因增加了抗氧化酶（超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶、谷胱甘肽S-转移酶）、天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶、中性蛋白酶和蛋白酶抑制剂的活性，以及尿酸、甘油三酯、胆固醇、葡萄糖和钙离子的浓度。经咖啡因处理的蜜蜂中酸性和碱性蛋白酶活性较低。经咖啡因处理的工蜂中肌酐和镁离子浓度较高，但仅在14日龄之前如此。咖啡因显著降低了老年蜜蜂的DNA甲基化水平。该化合物可被视为一种天然饮食补充剂，可增强蜜蜂对压力因素的抵抗力。我们的研究将增加把意大利蜜蜂用作老年学研究模式生物的可能性。
