Milto I V, Grishanova A Yu, Klimenteva T K, Suhodolo I V, Vasukov G Yu, Ivanova V V
Siberian State Medical University, Tomsk, 634050, Russia.
Biochemistry (Mosc). 2014 Nov;79(11):1245-54. doi: 10.1134/S0006297914110121.
The influence of modified nanosized magnetite (NSM) particles (magnetic microspheres coated with chitosan and magnetoliposomes) after a single intravenous infusion of their suspensions on iron metabolism in rats has been studied. Modern physical and chemical methods (X-ray fluorescence, dynamic light scattering, transmission electron microscopy) were used for standardization of the modified NSM particles (their size, structure, ζ-potential, and concentration were determined). Atomic emission spectroscopy was used to reveal the dynamics of iron content in rat liver, spleen, lungs, and kidneys during 120 days. Colorimetric and immunoturbidimetric methods were used to determine the concentrations of plasma iron and the proteins involved in its metabolism - ceruloplasmin, transferrin, and ferritin. Their dynamics throughout the experiments were studied.
研究了单次静脉输注改性纳米磁铁矿（NSM）颗粒（壳聚糖包被的磁性微球和磁脂质体）悬浮液后对大鼠铁代谢的影响。采用现代物理和化学方法（X射线荧光、动态光散射、透射电子显微镜）对改性NSM颗粒进行标准化（测定其大小、结构、ζ电位和浓度）。利用原子发射光谱法揭示大鼠肝脏、脾脏、肺和肾脏在120天内铁含量的动态变化。采用比色法和免疫比浊法测定血浆铁浓度以及参与其代谢的蛋白质——铜蓝蛋白、转铁蛋白和铁蛋白的浓度。研究了它们在整个实验过程中的动态变化。
