Departament de Fisicoquímica, Facultat de Farmàcia, Universitat de Barcelona, Avda. Joan XXIII, 08028 Barcelona, Catalonia, Spain.
Int J Pharm. 2011 Feb 28;405(1-2):181-7. doi: 10.1016/j.ijpharm.2010.11.044. Epub 2010 Dec 1.
Anionic ferrofluid was encapsulated in 200nm-diameter liposomes. The process involved phase-reverse evaporation followed by sequential extrusion. Magnetoliposomes were characterized by transmission electron microscopy, Doppler laser electrophoresis, SQUID magnetometry, dynamic light scattering and iron content by atomic absorption spectrophotometry. The absence of hysteresis of the magnetic power of particles at room temperature is characteristic of a material with superparamagnetic properties. The encapsulation efficiency was determined for several iron/phospholipid ratios, and this parameter ranged from 0.016 to 0.024mg iron per mmole of phospholipids, depending on the initial magnetite concentration. In comparison with magnetoliposomes that were obtained solely by extrusion, this method afforded significantly better encapsulation (P=0.0002). Magnetic particles were intravenously administered to healthy or inflammation-induced mice. After 1h, the content of iron was determined in exudates, liver, spleen and plasma. Magnetoliposomes accumulated in the exudates collected from the inflammation site, which suggests that these particles could be loaded with the drugs needed to treat some inflammatory processes.
阴离子铁磁流体被包裹在 200nm 直径的脂质体中。该过程涉及相逆转蒸发，然后是连续挤压。磁脂体通过透射电子显微镜、多普勒激光电泳、超导量子干涉磁强计、动态光散射和原子吸收分光光度法测定铁含量进行表征。室温下颗粒磁强的无滞后性是具有超顺磁性材料的特征。根据初始磁铁矿浓度，确定了几种铁/磷脂比的包封效率，该参数范围为 0.016 至 0.024mg 铁/每毫摩尔磷脂，这取决于初始磁铁矿浓度。与仅通过挤压获得的磁脂体相比，该方法提供了更好的包封效率（P=0.0002）。将磁性颗粒经静脉注射到健康或炎症诱导的小鼠体内。1 小时后，在渗出物、肝脏、脾脏和血浆中测定铁含量。磁脂体在炎症部位收集的渗出物中积累，这表明这些颗粒可以装载用于治疗某些炎症过程的药物。
