Department of Microbiology and Immunology, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Box 435, S-40530 Göteborg, Sweden.
Expert Rev Vaccines. 2015 Apr;14(4):551-60. doi: 10.1586/14760584.2015.996553. Epub 2014 Dec 26.
Enterotoxigenic Escherichia coli (ETEC) is a major cause of morbidity and mortality caused by diarrhea in children less than 5 years of age in low- and middle-income countries. Despite a wealth of research elucidating the mechanisms of disease, the immunological responses and vaccine development, ETEC is still relatively uncharacterized when it comes to regulation of virulence and detailed immune mechanisms. The recent emergence of next-generation sequencing now offers the possibility to screen genomes of ETEC strains isolated globally to identify novel vaccine targets in addition to those already established. In this review, we discuss how recent findings on ETEC genomics using novel sequencing techniques will aid in finding novel protective antigens that can be used in vaccine approaches.
肠毒素性大肠杆菌（ETEC）是导致低收入和中等收入国家 5 岁以下儿童腹泻发病率和死亡率的主要原因。尽管有大量研究阐明了疾病的发病机制、免疫反应和疫苗开发，但在调节毒力和详细的免疫机制方面，ETEC 的特征仍然相对不明显。最近出现的下一代测序技术现在提供了一种可能，可以筛选全球分离的 ETEC 菌株的基因组，以确定除已经确定的那些之外的新型疫苗靶点。在这篇综述中，我们讨论了使用新型测序技术对 ETEC 基因组学的最新发现如何有助于寻找可用于疫苗接种的新型保护性抗原。
